Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-11-04 DOI:10.1093/jambio/lxae259

Taya Tang, Yinzhu Chen, Zhongling Zhao, Qianyu Bai, Jørgen J Leisner, Tianlong Liu

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Abstract

Aims: To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains.

Methods and results: The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres.

Conclusions: This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.

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尼生素载体壳聚糖/海藻酸钠微球增强了尼生素对金黄色葡萄球菌的抗菌效果。

目的：开发并评估壳聚糖/海藻酸钠（CS/SA）微球作为针对金黄色葡萄球菌菌株的改进型抗菌给药系统：采用改良的油包水乳液交联法制备了微球，经扫描电子显微镜（SEM）和Zetasizer分析证实，微球为1-8 µm大小的球形颗粒，表面电荷为-7.92 ± 5.09 mV。尼生素的包封效率（EE）和负载能力（LC）分别为 87.60 ± 0.43% 和 1.99 ± 0.01%。48 小时的体外释放研究表明，尼生素的释放模式是受控的，可用 Korsmeyer-Peppas 模型来描述，在 37°C 和碱性 pH 条件下释放率较高。抗菌试验表明，与游离的尼生素相比，尼生素负载 CS/SA 微球的功效更强，最低抑菌浓度 (MIC) 值降低了 50%。共焦激光扫描显微镜（CLSM）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）显示，微球诱导了明显的细菌膜损伤和细胞破坏：本研究强调了尼生素载体 CS/SA 微球作为一种创新的抗菌给药系统的潜力，它具有更高的稳定性和对金黄色葡萄球菌的抗菌效果，解决了单独使用尼生素的局限性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.