Association of IL-6 G-174C (rs1800795) variant with the susceptibility to hepatocellular carcinoma in patients with chronic hepatitis.

Eman H Abuelnadar, Lamiaa M Ramadan, Hanaa Elsayed Shahin, Saleha Y M Alakilli, Eman Wahsh, Nanis S El-Beltagy, Eman T Salem, Abdelrahman S Hatata, Afaf M El-Said, Maha Alhelf
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Abstract

Aim: An ineffective immune response resulting from dysregulation of cytokine production might encourage viral persistence and cause chronic viral hepatitis to worsen. This study examined the relationship between alterations in interleukin-6 (IL-6) levels and the IL-6 - 174 G > C (rs1800795) polymorphism, as well as how this polymorphism affects the development and progression of chronic hepatitis brought on by hepatitis B (HBV) and hepatitis C (HCV) into hepatocellular carcinoma (HCC).

Patients and methods: Whole blood samples from 126 Egyptian patients with HCC (111 with HCV and 15 with HBV), as well as 126 age- and sex-matched healthy individuals, were used to extract DNA. Using PCR-based allele-specific amplification (ASA), the existence of the IL-6 G-174C polymorphism was investigated. Additionally, each participant's serum IL-6 levels were determined using an enzyme-linked immunosorbent assay (ELISA).

Results: The primary observations revealed that HCC patients had greater serum levels of IL-6 compared to the control groups (p < 0.001). Patients with the variant (CG and GG) genotype in the HCC group were found to have more disease severity indicated by higher levels of alpha-fetoprotein (AFP) and a higher ascites grade, as well as increased inflammatory activity as defined by higher levels of IL-6 and C-reactive protein (CRP) (p < 0.001 for both) in comparison to patients with the wild-type (CC) genotype (p < 0.001 and p = 0.002, respectively).

Conclusion: The rs1800795 SNP in the IL-6 gene was associated with increased inflammatory activity and high levels of IL-6, indicating that this SNP may play a role in the development of HCC in Egyptian patients with chronic viral hepatitis.

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IL-6 G-174C (rs1800795) 变异与慢性肝炎患者肝细胞癌易感性的关系。
目的:细胞因子分泌失调导致的无效免疫反应可能会促使病毒持续存在并导致慢性病毒性肝炎恶化。本研究探讨了白细胞介素-6(IL-6)水平的变化与 IL-6 - 174 G > C(rs1800795)多态性之间的关系,以及该多态性如何影响乙型肝炎(HBV)和丙型肝炎(HCV)导致的慢性肝炎发展为肝细胞癌(HCC):从 126 名埃及 HCC 患者(111 名 HCV 患者和 15 名 HBV 患者)以及 126 名年龄和性别匹配的健康人的全血样本中提取 DNA。利用基于 PCR 的等位基因特异性扩增(ASA)技术,研究了 IL-6 G-174C 多态性的存在。此外,还使用酶联免疫吸附试验(ELISA)测定了每位参与者的血清 IL-6 水平:主要观察结果显示,与对照组相比,HCC 患者血清中的 IL-6 水平更高(p 结论:HCC 患者血清中的 IL-6 水平高于对照组:IL-6基因中的rs1800795 SNP与炎症活动增加和高水平的IL-6有关,表明该SNP可能在埃及慢性病毒性肝炎患者的HCC发病中发挥作用。
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来源期刊
CiteScore
3.50
自引率
0.00%
发文量
46
审稿时长
11 weeks
期刊介绍: As the official publication of the National Cancer Institute, Cairo University, the Journal of the Egyptian National Cancer Institute (JENCI) is an open access peer-reviewed journal that publishes on the latest innovations in oncology and thereby, providing academics and clinicians a leading research platform. JENCI welcomes submissions pertaining to all fields of basic, applied and clinical cancer research. Main topics of interest include: local and systemic anticancer therapy (with specific interest on applied cancer research from developing countries); experimental oncology; early cancer detection; randomized trials (including negatives ones); and key emerging fields of personalized medicine, such as molecular pathology, bioinformatics, and biotechnologies.
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