Utility of clinical, laboratory, and lymph node MYD88 L265P mutation in risk assessment of diffuse large B-cell lymphoma patients.

Ahmed Talaat Hanbal, Shaimaa El-Ashwah, Ahmed E Eladl, Sameh Shamaa, Layla M Saleh
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Abstract

Background: Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma and is characterized by heterogeneity in biology and clinical behavior. Mutations in the myeloid differentiation primary response 88 (MYD88) are found in different lymphoproliferative disorders and are associated with variable clinical and prognostic impact.

Aim: To investigate the frequency of MYD88 L265P mutation and its clinical impact in a cohort of Egyptian DLBCL patients.

Methods: FFPE lymph node samples from 87 DLBCL patients (46 males / 41 females; median age, 58 years) were included and analyzed for MYD88 L265P by an allele-specific PCR.

Results: MYD88 L265P mutations were found in 52 patients (59.8%) out of 87 DLBCL cases. Patients with L265 mutation were significantly younger than non-mutated patients (p = 0.022). None of the patients with the L265P mutation showed a significant association with the clinical parameters of DLBCL. Interestingly, MYD88 L265 mutated patients were found to be significantly correlated with HCV infection (p = 0.037). The median follow-up time across the entire cohort was 26 months. Univariate analysis showed that overall survival (OS) was affected by gender, LDH level, and CNS-IPI scoring (p = 0.048, 0.008, and 0.046, respectively), while disease-free survival (DFS) was affected by B symptoms and LDH level (p =  < 0.000 and 0.02, respectively). However, the MYD88 mutation status and other prognostic factors showed no association with OS or DFS.

Conclusions: Our findings indicate a high frequency of MYD88 L265P mutations in our study population and not associated with prognostic markers or the outcome of the disease.

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弥漫大 B 细胞淋巴瘤患者风险评估中临床、实验室和淋巴结 MYD88 L265P 突变的实用性。
背景:弥漫大B细胞淋巴瘤(DLBCL)是一种侵袭性非霍奇金淋巴瘤,其生物学和临床表现具有异质性。目的:研究埃及 DLBCL 患者队列中 MYD88 L265P 突变的频率及其临床影响:纳入87名DLBCL患者(46名男性/41名女性;中位年龄58岁)的FFPE淋巴结样本,并通过等位基因特异性PCR分析MYD88 L265P:结果:87例DLBCL患者中有52例(59.8%)发现了MYD88 L265P突变。L265突变患者明显比未突变患者年轻(P = 0.022)。L265P突变患者均未显示出与DLBCL临床参数的显著关联。有趣的是,MYD88 L265突变患者与HCV感染有显著相关性(p = 0.037)。整个队列的中位随访时间为26个月。单变量分析显示,总生存期(OS)受性别、LDH 水平和 CNS-IPI 评分的影响(p = 0.048、0.008 和 0.046,分别为 0.048、0.008 和 0.046),而无病生存期(DFS)受 B 症状和 LDH 水平的影响(p = 0.008、0.008 和 0.046):我们的研究结果表明,在我们的研究人群中,MYD88 L265P 突变的频率很高,但与预后标志物或疾病结果无关。
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来源期刊
CiteScore
3.50
自引率
0.00%
发文量
46
审稿时长
11 weeks
期刊介绍: As the official publication of the National Cancer Institute, Cairo University, the Journal of the Egyptian National Cancer Institute (JENCI) is an open access peer-reviewed journal that publishes on the latest innovations in oncology and thereby, providing academics and clinicians a leading research platform. JENCI welcomes submissions pertaining to all fields of basic, applied and clinical cancer research. Main topics of interest include: local and systemic anticancer therapy (with specific interest on applied cancer research from developing countries); experimental oncology; early cancer detection; randomized trials (including negatives ones); and key emerging fields of personalized medicine, such as molecular pathology, bioinformatics, and biotechnologies.
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