Prolongation of seed viability and grain quality in rice by editing OsLOX1 using CRISPR/Cas9.

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2024-10-12 eCollection Date: 2024-10-01 DOI:10.1007/s11032-024-01506-4

Changling Mou, Yaping Chen, Ping Zhang, Qikai Tong, Ziyan Zhu, Tengfei Ma, Ping Wang, Kai Fu, Cheng Chen, Yunshuai Huang, Fulin Zhang, Qixian Hao, Min Zhang, Shijia Liu, Ling Jiang, Jianmin Wan

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Abstract

Deterioration of rice (Oryza sativa L.) affects grain quality and seed viability during storage. Lipoxygenase (LOX), a key enzyme in lipid metabolism, directly affects the rate of ageing. Here, we found that knock-out of lipoxygenase gene OsLOX1 by CRISPR/Cas9 delayed loss of seed viability and quality. Transcriptome analysis showed that during storage, OsLOX1 affected transcription of multiple genes, including genes related to lipid metabolism and antioxidant pathways such as phosphatase and acetaldehyde dehydrogenase, which may regulate the seed storability. The genes significantly down- and up-regulated only in Ningjing 4 after NA for 13 months and 3 days of AA suggesting that OsLOX1 likely promoted seed viability in rice by balancing ageing and storage related genes, and regulated the seed storability through the amino acid synthesis and metabolic pathways. Moreover, knock-out of OsLOX1 without CRISPR/Cas9 not only improved the seed viability, but also had little impact on agronomic traits. More importantly, the OsLOX1 knock-out lines were approved in 2019 (Agricultural Foundation of China Report No. 770). Collectively, our study showed that knock-out of OsLOX1 is beneficial for prolongation of seed viability and can be directly applied to agricultural production.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01506-4.

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利用 CRISPR/Cas9 编辑 OsLOX1，延长水稻种子活力和谷物品质。

水稻（Oryza sativa L.）在储藏期间的老化会影响谷物品质和种子活力。脂氧合酶（LOX）是脂质代谢的关键酶，直接影响老化速度。在这里，我们发现通过 CRISPR/Cas9 基因敲除脂氧合酶基因 OsLOX1 可以延缓种子活力和质量的损失。转录组分析表明，在贮藏过程中，OsLOX1会影响多个基因的转录，包括与脂质代谢和抗氧化途径相关的基因，如磷酸酶和乙醛脱氢酶，这些基因可能会调控种子的贮藏性。结果表明，OsLOX1可能通过平衡老化和贮藏相关基因促进水稻种子活力，并通过氨基酸合成和代谢途径调控种子贮藏性。此外，不使用 CRISPR/Cas9 基因敲除 OsLOX1 不仅能提高种子活力，而且对农艺性状影响不大。更重要的是，OsLOX1基因敲除品系于2019年获得批准（中国农业基金报告第770号）。总之，我们的研究表明，敲除OsLOX1有利于延长种子活力，可直接应用于农业生产：在线版本包含补充材料，见 10.1007/s11032-024-01506-4。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.