Integrated analysis of metabolome and microbiome in a rat model of perimenopausal syndrome.

IF 5 2区 生物学 Q1 MICROBIOLOGY mSystems Pub Date : 2024-11-19 Epub Date: 2024-10-21 DOI:10.1128/msystems.00623-24
Yanqiu Wei, Juanjuan Shi, Jianhua Wang, Zongyan Hu, Min Wang, Wen Wang, Xiujuan Cui
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Abstract

The objectives of this study are to examine the disparities in serum and intestinal tissue metabolites between a perimenopausal rat model and control rats and to analyze the diversity and functionality of intestinal microorganisms to determine the potential correlation between intestinal flora and metabolites. We established a rat model of perimenopausal syndrome (PMS) and performed an integrated analysis of metabolome and microbiome. Orthogonal partial least-squares discriminant analysis scores and replacement tests indicated distinct separations of anion and cation levels between serum and intestinal samples of the model and control groups. Furthermore, lipids and lipid-like molecules constituted the largest percentage of HMDB compounds in both serum and intestinal tissues, followed by organic acids and derivatives, and organoheterocyclic compounds, with other compounds showing significant variability. Moreover, analysis of diversity and functional enrichment of the intestinal microflora and correlation analysis with metabolites revealed significant variability in the composition of the intestinal flora between the normal control and perimenopausal groups, with these differentially expressed intestinal flora strongly correlated with their metabolites. The findings of this study are expected to contribute to understanding the indications and contraindications for estrogen application in perimenopausal women and to aid in the development of appropriate therapeutic agents.

Importance: In this work, we employed 16S ribosomal RNA gene sequencing to analyze the gut microbes in stool samples. In addition, we conducted an ultra-high-performance liquid chromatography-tandem mass spectrometry-based metabolomics approach on gut tissue and serum obtained from rats with perimenopausal syndrome (PMS) and healthy controls. By characterizing the composition and metabolomic properties of gut microbes in PMS rats, we aim to enhance our understanding of their role in women's health, emphasizing the significance of regulating gut microbes in the context of menopausal women's well-being. We aim to provide a theoretical basis for the prevention and treatment of PMS in terms of gut microflora as well as metabolism.

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围绝经期综合征大鼠模型中代谢组和微生物组的综合分析。
本研究的目的是检测围绝经期大鼠模型与对照组大鼠之间血清和肠道组织代谢物的差异,并分析肠道微生物的多样性和功能性,以确定肠道菌群与代谢物之间的潜在相关性。我们建立了围绝经期综合征(PMS)大鼠模型,并对代谢组和微生物组进行了综合分析。正交偏最小二乘判别分析得分和置换检验表明,模型组和对照组的血清和肠道样本中阴离子和阳离子水平有明显的分离。此外,在血清和肠道组织中,脂质和类脂质分子在 HMDB 化合物中所占的比例最大,其次是有机酸及其衍生物和有机杂环化合物,其他化合物则表现出显著的差异性。此外,肠道微生物菌群的多样性和功能富集分析以及与代谢物的相关性分析表明,正常对照组和围绝经期组的肠道菌群组成存在显著差异,这些差异表达的肠道菌群与其代谢物密切相关。本研究的结果有望帮助了解围绝经期妇女应用雌激素的适应症和禁忌症,并有助于开发适当的治疗药物:在这项工作中,我们采用 16S 核糖体 RNA 基因测序分析了粪便样本中的肠道微生物。此外,我们还对围绝经期综合征(PMS)大鼠和健康对照组大鼠的肠道组织和血清进行了基于超高效液相色谱-串联质谱的代谢组学研究。通过分析围绝经期综合征大鼠肠道微生物的组成和代谢组学特性,我们希望加深对它们在女性健康中所起作用的了解,强调调节肠道微生物对更年期女性健康的重要意义。我们的目标是从肠道微生物区系和新陈代谢方面为经前综合征的预防和治疗提供理论依据。
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来源期刊
mSystems
mSystems Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
10.50
自引率
3.10%
发文量
308
审稿时长
13 weeks
期刊介绍: mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.
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