Facile fabrication of nano-bioactive glass functionalized blended hydrogel with nucleus pulposus-derived MSCs to improve regeneration potential in treatment of disc degeneration by in vivo rat model.

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-10-15 DOI:10.1016/j.nano.2024.102790
Chong Bian, Guangnan Chen, Xiangyang Cheng, Huijie Gu, Zhongyue Huang, Kaifeng Zhou
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引用次数: 0

Abstract

Orthopaedic medicine often treats intervertebral disc degeneration (IVDD), which is caused by nucleus pulposus (NP) tissue damage and mechanical stress. Bioactive glasses (BGs), widely used for bone regeneration, can incorporate therapeutic ions into their network. Manganese (Mn) activates human osteoblast integrins, proliferation, and spreading. The CMnBGNPs-NPMSCs are carboxymethyl cellulose hydrogels functionalized with MnBGsNPs and NP-derived mesenchymal stem cells to treat IVDD. To ensure stability and biocompatibility of CMnBGNPs-NPMSCs were characterized for rheological properties like gelation time and swelling ratio. Gene expression analysis of PAX1, FOXF1, CA12, HBB, and OVOS2 via qRT-PCR further assessed the hydrogel's characteristics. Rat models with induced IVDD had hydrogel-MSC composite injected into their intervertebral discs for in vivo studies. Histological examination, immunohistochemical staining for inflammation and disc regeneration markers, and disc height assessments assessed therapeutic efficacy. CMnBGNPs-NPMSCs show promising results for IVDD treatment, offering a novel therapeutic strategy with clinical implications for degenerative disc diseases.

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利用髓核间充质干细胞轻松制备纳米生物活性玻璃功能化混合水凝胶,通过体内大鼠模型提高治疗椎间盘退行性变的再生潜力。
骨科医学经常治疗由髓核组织损伤和机械应力引起的椎间盘变性(IVDD)。广泛用于骨再生的生物活性玻璃(BGs)可以在其网络中加入治疗离子。锰(Mn)可激活人类成骨细胞整合素、增殖和扩散。CMnBGNPs-NPMSCs 是一种羧甲基纤维素水凝胶,其中含有功能化的 MnBGsNPs 和 NP 衍生间充质干细胞,可用于治疗 IVDD。为确保 CMnBGNPs-NPMSCs 的稳定性和生物相容性,研究人员对其流变特性(如凝胶时间和膨胀率)进行了表征。通过 qRT-PCR 对 PAX1、FOXF1、CA12、HBB 和 OVOS2 进行基因表达分析,进一步评估了水凝胶的特性。将水凝胶-间充质干细胞复合材料注射到诱发 IVDD 的大鼠椎间盘中进行体内研究。组织学检查、炎症和椎间盘再生标记物的免疫组化染色以及椎间盘高度评估评估了疗效。CMnBGNPs-NPMSCs在IVDD治疗中显示出良好的效果,为椎间盘退行性疾病提供了一种具有临床意义的新型治疗策略。
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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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