Differentially expressed lncRNAs in SOD1G93A mice skeletal muscle: H19, Myhas and Neat1 as potential biomarkers in amyotrophic lateral sclerosis.

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Open Biology Pub Date : 2024-10-01 Epub Date: 2024-10-16 DOI:10.1098/rsob.240015
Tresa López-Royo, Laura Moreno-Martínez, Pilar Zaragoza, Alberto García-Redondo, Raquel Manzano, Rosario Osta
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Abstract

Amyotrophic lateral sclerosis (ALS) is a devastating neuromuscular disease characterized by progressive motor function and muscle mass loss. Despite extensive research in the field, the underlying causes of ALS remain incompletely understood, contributing to the absence of specific diagnostic and prognostic biomarkers and effective therapies. This study investigates the expression of long-non-coding RNAs (lncRNAs) in skeletal muscle as a potential source of biomarkers and therapeutic targets for the disease. The expression profiles of 12 lncRNAs, selected from the literature, were evaluated across different disease stages in tissue and muscle biopsies from the SOD1G93A transgenic mouse model of ALS. Nine out of the 12 lncRNAs were differentially expressed, with Pvt1, H19 and Neat1 showing notable increases in the symptomatic stages of the disease, and suggesting their potential as candidate biomarkers to support diagnosis and key players in muscle pathophysiology in ALS. Furthermore, the progression of Myhas and H19 RNA levels across disease stages correlated with longevity in the SOD1G93A animal model, effectively discriminating between long- and short-term survival individuals, thereby highlighting their potential as prognostic indicators. These findings underscore the involvement of lncRNAs, especially H19 and Myhas, in ALS pathophysiology, offering novel insights for diagnostic, prognostic and therapeutic targets.

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SOD1G93A小鼠骨骼肌中差异表达的lncRNA:H19、Myhas和Neat1作为肌萎缩侧索硬化症的潜在生物标记物。
肌萎缩性脊髓侧索硬化症(ALS)是一种以进行性运动功能和肌肉质量丧失为特征的破坏性神经肌肉疾病。尽管在该领域开展了广泛的研究,但对肌萎缩性脊髓侧索硬化症的根本原因仍不完全清楚,导致缺乏特定的诊断和预后生物标志物以及有效的疗法。本研究调查了骨骼肌中长非编码 RNA(lncRNA)的表达情况,以此作为该疾病的潜在生物标志物和治疗靶点。研究人员从文献中筛选出12种lncRNAs,评估了它们在SOD1G93A转基因小鼠ALS模型的组织和肌肉活检中不同疾病阶段的表达谱。在这12个lncRNA中,有9个存在差异表达,其中Pvt1、H19和Neat1在疾病的症状期明显增加,这表明它们有可能成为支持诊断的候选生物标记物和ALS肌肉病理生理学的关键参与者。此外,在SOD1G93A动物模型中,Myhas和H19 RNA水平在疾病各阶段的进展与寿命相关,可有效区分长期和短期存活的个体,从而突出了它们作为预后指标的潜力。这些发现强调了 lncRNAs,尤其是 H19 和 Myhas 参与 ALS 病理生理学,为诊断、预后和治疗目标提供了新的见解。
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来源期刊
Open Biology
Open Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
10.00
自引率
1.70%
发文量
136
审稿时长
6-12 weeks
期刊介绍: Open Biology is an online journal that welcomes original, high impact research in cell and developmental biology, molecular and structural biology, biochemistry, neuroscience, immunology, microbiology and genetics.
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