Antibacterial and Anticancer Properties of Endophenazines from Streptomyces prasinus ZO16, an Endophyte in Zingiber officinale Rosc.

Q3 Agricultural and Biological Sciences Pakistan Journal of Biological Sciences Pub Date : 2024-08-01 DOI:10.3923/pjbs.2024.469.478
Thongchai Taechowisan, Thanaporn Chuen-Im, Waya S Phutdhawong
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Abstract

<b>Background and Objective:</b> This study investigated a bacterial strain, ZO16, isolated from ginger (<i>Zingiber officinale</i>) roots. Analysis of its 16S ribosomal DNA (rDNA), along with chemical and physical properties, revealed it to be <i>Streptomyces prasinus</i>. This study aimed to isolate and characterize the main bioactive compounds from ZO16, evaluating their antibacterial and anticancer properties. <b>Materials and Methods:</b> Techniques like column chromatography and thin-layer chromatography (TLC) were used to purify the key compounds from ZO16's culture extract. Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry were utilized to confirm the identities of the purified compounds as endophenazine A (compound 1) and endophenazine B (compound 2). The antibacterial and anticancer properties of these compounds were then evaluated. <b>Results:</b> The isolated compounds displayed antibacterial activity against <i>Staphylococcus aureus</i> ATCC 25923 and Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA). The minimum inhibitory concentration (MIC) of the isolated compounds against bacteria ranged from 8 to 32 μg/mL, while the minimum bactericidal concentration (MBC) was between 32 and 128 μg/mL. These compounds exhibited effectiveness against tested cancer cells with IC<sub>50</sub> values ranging from 30.40 to 32.51 μg/mL for cervical cancer (HeLa), 78.32 to 86.45 μg/mL for liver cancer (HepG2) and 23.41 to 28.26 μg/mL for breast cancer (MDA-MB-231) cells. However, these compounds also showed moderate toxicity towards non-cancerous Vero cells (IC<sub>50</sub> = 317.44-328.63 μg/mL). <b>Conclusion:</b> The findings of this study suggest that <i>Streptomyces prasinus</i> strain ZO16 produces compounds with antibacterial and anticancer properties. Further investigation of these compounds has the potential to contribute to the development of improved methods for controlling and treating bacterial infections and some cancers.

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来自 Zingiber officinale Rosc.内生菌 Streptomyces prasinus ZO16 的内啡肽的抗菌和抗癌特性
<b>背景与目的:</b>本研究调查了从生姜(<i>Zingiber officinale</i>)根中分离出的细菌菌株ZO16。对其 16S 核糖体 DNA(rDNA)以及化学和物理性质的分析表明,它是 <i>Streptomyces prasinus</i>。<b>Materials and Methods:</b> 采用柱层析和薄层层析(TLC)等技术从 ZO16 的培养提取物中纯化关键化合物。利用核磁共振(NMR)光谱和质谱法确认了纯化化合物的身份,即内啡肽 A(化合物 1)和内啡肽 B(化合物 2)。结果:</b> 分离出的化合物对金黄色葡萄球菌<i>ATCC 25923 和耐甲氧西林金黄色葡萄球菌<i>(MRSA)具有抗菌活性。分离出的化合物对细菌的最小抑菌浓度(MIC)为 8 至 32 μg/mL,最小杀菌浓度(MBC)为 32 至 128 μg/mL。这些化合物对测试的癌细胞有效,对宫颈癌(HeLa)细胞的 IC<sub>50</sub> 值为 30.40 至 32.51 μg/mL,对肝癌(HepG2)细胞的 IC<sub>50</sub> 值为 78.32 至 86.45 μg/mL,对乳腺癌(MDA-MB-231)细胞的 IC<sub>50</sub> 值为 23.41 至 28.26 μg/mL。不过,这些化合物对非癌 Vero 细胞也显示出中等毒性(IC<sub>50</sub> = 317.44-328.63 μg/mL)。 <b>结论:</b> 这项研究的结果表明,<i>Streptomyces prasinus</i> 菌株 ZO16 能产生具有抗菌和抗癌特性的化合物。对这些化合物的进一步研究有可能有助于开发出更好的方法来控制和治疗细菌感染和某些癌症。
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来源期刊
Pakistan Journal of Biological Sciences
Pakistan Journal of Biological Sciences Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.90
自引率
0.00%
发文量
102
期刊介绍: Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.
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