Novel miRNA biomarkers for alveolar echinococcosis: sequencing and clinical validation.

IF 2.1 3区 医学 Q2 PARASITOLOGY Parasitology Pub Date : 2024-10-18 DOI:10.1017/S0031182024001367
Jideng Ma, Zian Li, Lanmin Liu, Xiaoqin Luo, Xiaoya Ma, Yumei Zhang, Lei Jiang, Xiangren A
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Abstract

This study aimed to explore extracellular microRNA derived from Echinococcus multilocularis (EM) in the plasma of patients with alveolar echinococcosis (AE) and assess its potential as a diagnostic biomarker. EM-derived miRNAs were identified in plasma samples from 20 AE patients through miRNA sequencing. Three novel miRNA molecules (emu-miR-novel 1, 2 and 3) were predicted through bioinformatic analysis to elucidate their chromosomal locations, secondary structures and precursor forms. Subsequently, plasma samples from 30 AE patients and 30 controls were utilized to establish an assay via stem-loop reverse transcription PCR, optimizing primers, reaction systems, and conditions to assess cross-reactivity and sensitivity. Clinical validation revealed that emu-miR-novel 1 had the highest diagnostic accuracy, with an area under the curve (AUC) of 0.8994, a P value of less than 0.0001, a sensitivity of 83.3%, and a specificity of 86.7%. Statistically significant differences were observed between the groups for emu-miR-novel 1 (P < 0.05), whereas emu-miR-novel 2 and 3 showed AUC values of 0.7922 and 0.6883, with P values of 0.0001 and 0.012, respectively, indicating no significant difference between groups (P > 0.05). Furthermore, the assay showed no cross-reactivity with samples from 18 common viruses, 4 parasitic infections, and miRNAs from AE sequenced from 8 species, confirming its high specificity. Emu-miR-novel 1 exhibited a sensitivity of 1 femtomolar. Emu-miR-novel 1 holds promise as a key diagnostic tool for AE, offering a novel perspective and approach for disease diagnosis.

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肺泡棘球蚴病的新型 miRNA 生物标记物:测序和临床验证。
本研究旨在探索肺泡棘球蚴病(AE)患者血浆中来自多房棘球蚴(EM)的细胞外microRNA,并评估其作为诊断生物标志物的潜力。通过miRNA测序,从20例AE患者的血浆样本中鉴定出em来源的miRNA。通过生物信息学分析预测了三个新的miRNA分子(emu-miR-novel 1、2和3),阐明了它们的染色体位置、二级结构和前体形式。随后,利用30例AE患者和30例对照组的血浆样本,通过茎环反转录PCR建立了一种检测方法,优化了引物、反应系统和条件,以评估交叉反应性和敏感性。临床验证显示,emu-miR-novel 1具有最高的诊断准确率,曲线下面积(AUC)为0.8994,P值小于0.0001,敏感性为83.3%,特异性为86.7%。emu-miR-novel 1组间差异有统计学意义(P < 0.05), emu-miR-novel 2和3组间AUC值分别为0.7922和0.6883,P值分别为0.0001和0.012,组间差异无统计学意义(P < 0.05)。此外,该方法与18种常见病毒、4种寄生虫感染和8种AE测序的mirna无交叉反应性,证实了其高特异性。Emu-miR-novel 1的敏感性为1飞摩尔。Emu-miR-novel 1有望成为AE的关键诊断工具,为疾病诊断提供新的视角和方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Parasitology
Parasitology 医学-寄生虫学
CiteScore
4.80
自引率
4.20%
发文量
280
审稿时长
3-8 weeks
期刊介绍: Parasitology is an important specialist journal covering the latest advances in the subject. It publishes original research and review papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in parasite biochemistry, molecular biology and genetics, ecology and epidemiology in the context of the biological, medical and veterinary sciences. Included in the subscription price are two special issues which contain reviews of current hot topics, one of which is the proceedings of the annual Symposia of the British Society for Parasitology, while the second, covering areas of significant topical interest, is commissioned by the editors and the editorial board.
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