Effect of silver nanoparticles on donkey sperm parameters and ultrastructure.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14662

Verónica Pérez, Francisco Crespo, Angela I López, Soledad Cárdenas, María José Bautista, Manuel Hidalgo, Jesus Dorado, Isabel Ortiz

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Abstract

The aim of this study was to determine the effect of silver nanoparticles (AgNPs) on donkey sperm parameters and ultrastructure. AgNPs were synthesized, purified and resuspended in the extender. Nine frozen-thawed donkey sperm samples were exposed to different concentrations of AgNPs (0, 1.25, 2.5, 5, 12.5, 25 and 50 μg/mL). Sperm parameters: total (TMOT, %) and progressive (PMOT, %) sperm motility, plasma (LIVE, %) and acrosomal membrane integrity (AIS, %), and sperm morphology (MORF, %) were evaluated immediately after AgNPs exposure (T0) and after 2 h of incubation (T2). The interaction beween AgNPs and spermatozoa was visualized by transmission electron microscopy (TEM). At T0, sperm motility and AIS were reduced (p < .05) when using concentrations ≥50 and ≥25 μg/mL, respectively. At T2, sperm motility and LIVE were significantly decreased (p < .05) in concentrations ≥25 and ≥50 μg/mL, respectively. TEM analysis revealed nanoparticle adhesion to the acrosomal region of the plasma membrane. In conclusion, AgNPs at concentrations ≥25 μg/mL impair motility, acrosome and plasma membrane integrity of donkey sperm, which may be mediated by adhesion to the acrosomal region of the sperm surface membrane.

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纳米银颗粒对驴精子参数和超微结构的影响

本研究旨在确定银纳米粒子（AgNPs）对驴精子参数和超微结构的影响。AgNPs经合成、纯化并重悬于扩展剂中。将 9 份冷冻解冻的驴精子样本暴露于不同浓度的 AgNPs（0、1.25、2.5、5、12.5、25 和 50 μg/mL）。精子参数：精子总活力（TMOT，%）和进步活力（PMOT，%）、精浆（LIVE，%）和顶体膜完整性（AIS，%）以及精子形态（MORF，%）分别在接触 AgNPs 后立即（T0）和培养 2 小时后（T2）进行评估。通过透射电子显微镜（TEM）观察 AgNPs 与精子之间的相互作用。在 T0 阶段，精子活力和 AIS 均下降（p

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.