In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14654

Gabriela Garrappa, Cristina Martínez-López, María Jiménez-Movilla, Francisco A García-Vázquez

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Abstract

Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP.

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猪精子与功能化超顺磁性纳米粒子的体外接触。

近几十年来，纳米技术及其应用取得了长足的进步，为包括生殖在内的各个领域做出了贡献。本研究介绍了一种新方法，用与输卵管蛋白（OVGP1，Ov）结合的纳米颗粒（NPs）标记猪卵母细胞，用于辅助生殖技术（ART）。尽管发展前景广阔，但配子中的 NP 毒性问题仍值得深入研究。本研究旨在评估功能化 NP（NPOv）对精子功能的影响。公猪精子与 NPOv 在两种培养基中分别共孵育 0、0.5 和 1 小时：BTS（精液稀释和保存）和 TALP（精子获能和体外受精-IVF）。在 TALP 培养基中，精子质量参数（存活率、活力和运动学）无显著差异（p > .05）。在 BTS 中，虽然一些动力学参数发生了变化，但运动能力、渐进运动能力和存活率仍未受到影响（p > .05）。此外，NPs 在卵母细胞透明带 (ZP) 上的存在并不影响精子附着（p > .05）。总之，公猪精子体外暴露于体外受精培养基中或附着在成熟卵母细胞透明带表面的 OVGP1 功能化 NPs 不会损害精子的功能，包括它们与透明带的结合能力。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.