Media viscosity affects post-thaw ram sperm rheotactic behaviour.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14644

A Martín-Maestro, L Abril-Parreño, A J Soler, S Fair

引用次数: 0

Abstract

The aim of this experiment was to assess the effect of media viscosity on ram sperm motility, kinematics and rheotaxis in vitro by using methylcellulose as a media thickener. Frozen-thawed semen of three rams was thawed and diluted in Tyrode's albumin lactate pyruvate (TALP) media supplemented with 0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6% and 0.7% w/v of methylcellulose. Sperm motility and kinematic characteristics were analysed using computer-assisted sperm analysis (CASA). The rheotactic behaviour was assessed in a microfluidic channel, and the number of spermatozoa that passed the 10 mm point of a microfluidic channel over a 2min period against a flow rate of 30 μm/sec was assessed. The use of media with higher viscosity (higher levels of methylcellulose) resulted in significantly lower (p < .05) sperm motility and kinematic parameters. Moreover, higher levels of methylcellulose reduced (p < .05) the number of spermatozoa that exhibited positive rheotaxis. In conclusion, viscosity affected the kinematic properties and rheotactic behaviour of ram sperm.

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介质粘度影响公羊精子解冻后的流变行为。

本实验的目的是通过使用甲基纤维素作为培养基增稠剂，评估培养基粘度对体外公羊精子活力、运动学和流变学的影响。将三只公羊冷冻解冻的精液解冻并稀释在添加了 0%、0.1%、0.2%、0.3%、0.4%、0.5%、0.6% 和 0.7% w/v 甲基纤维素的泰罗德白蛋白乳酸丙酮酸（TALP）培养基中。使用计算机辅助精子分析（CASA）分析了精子的运动性和运动特征。在微流体通道中对流变行为进行了评估，并评估了在 30 微米/秒的流速下，2 分钟内通过微流体通道 10 毫米点的精子数量。使用粘度较高的培养基（甲基纤维素含量较高）可显著降低（p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.