GW3, encoding a member of the P450 subfamily, controls grain width by regulating the GA4 content in spikelets of rice (Oryza sativa L.).

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-10-19 DOI:10.1007/s00122-024-04751-5
Xiaojing Dang, Qing Xu, Yulong Li, Shaojie Song, Changmin Hu, Chunyu Jing, Ying Zhang, Dezheng Wang, Delin Hong, Jianhua Jiang
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Abstract

Key message: A stable QTL, GW3, controlling grain width was identified in two populations. Its causal gene LOC_Os03g04680 was verified by gene-based haplotype analysis, expression analysis, gene knockout and complementation transgenic tests. Grain width (GW) is one of the key traits affecting grain size and determines grain yield and appearance quality in rice. Mining gene loci and elite alleles controlling GW is necessary. The GW phenotypes of the two populations were investigated in three environments, which showed abundant phenotypic variation. GW3, encoding a P450 subfamily protein, was identified and validated as a causal gene by gene-based haplotype analysis, expression analysis, gene knockout and complementation transgenic tests. The accessions with large GW values had high gene expression levels. In addition, the GW of the accessions with the GG allele was significantly greater than that of the accessions with the AA allele. The Hap 1 and Hap 3 were identified as elite haplotypes, which can increase GW. The expression levels of OsKO1, OsGA3ox1, OsGA20ox1 and OsGA20ox2 in the young panicle of A7444 were significantly greater than those in the young panicle of the mutants, indicating that GW3 may be involved in the gibberellins (GA) biosynthesis pathway to regulate GW. GA4 content detection and electron scanning analysis revealed that GA4 regulates GW by affecting glume cell size. These results provide new insights for studying the genetic mechanism of rice GW and provide a material basis for breeding high-yield rice varieties.

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编码 P450 亚家族成员的 GW3 通过调节水稻(Oryza sativa L.)小穗中 GA4 的含量来控制粒宽。
关键信息在两个群体中发现了控制粒宽的稳定 QTL GW3。通过基于基因的单倍型分析、表达分析、基因敲除和互补转基因试验验证了其致病基因LOC_Os03g04680。粒宽(GW)是影响水稻籽粒大小的关键性状之一,决定着水稻的籽粒产量和外观品质。挖掘控制粒宽的基因位点和精英等位基因是必要的。在三个环境中对两个群体的 GW 表型进行了研究,结果表明表型差异很大。通过基于基因的单倍型分析、表达分析、基因敲除和互补转基因试验,确定并验证了编码 P450 亚家族蛋白的 GW3 为致病基因。GW值大的基因序列具有较高的基因表达水平。此外,具有 GG 等位基因的种群的 GW 值明显高于具有 AA 等位基因的种群。Hap 1 和 Hap 3 被鉴定为精英单倍型,可提高 GW 值。A7444幼苗圆锥花序中OsKO1、OsGA3ox1、OsGA20ox1和OsGA20ox2的表达水平明显高于突变体幼苗圆锥花序中的表达水平,表明GW3可能参与赤霉素(GA)生物合成途径,调控GW。GA4含量检测和电子扫描分析表明,GA4通过影响颖果细胞大小来调控GW。这些结果为研究水稻GW的遗传机制提供了新的见解,为培育高产水稻品种提供了物质基础。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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