Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-21 DOI:10.1017/S0967199424000261

Ali Cihan Taskin, Ahmet Kocabay, Seref Gul, Gizem Nur Sahin, Sercin Karahuseyinoglu, I Halil Kavakli, Ibrahim Sogut

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Abstract

Boric acid (BA) is an important mineral for plants, animals and humans that assists metabolic function and has both positive and negative effects on biological systems. The present study aimed to investigate the effects of different concentrations of BA added to the culture media, the quality and in vitro development potential of mouse embryos. Superovulated C57Bl6/6j female mice were sacrificed ∼18 hours after human chorionic gonadotropin (hCG) injection. Single-cell-stage embryos were collected from the oviduct, divided into experiment groups and cultured in embryo medium with supplemented BA+ in 5% CO₂ at 37 °C until 96 hours at the blastocyst stage. The blastocyst development rates of 0, 1.62 × 10^-1, 1.62 × 10^-2, 1.62 × 10^-3 and 1.62 × 10^-4 µM BA were 51.52%, 73.47%, 77.36% and 81.13%, respectively. The in vitro development rates were significantly higher in the 1.62 × 10^-3 (p < 0.05) and 1.62 × 10^-4 µM BA groups than in the control group (p < 0.001). These results indicated that low BA doses influenced embryo development by positively affecting in vitro development rates, embryo cell numbers, biochemical parameters and development at the molecular level by pluripotent and antioxidant genes. Therefore, BA seems to play an important role on in vitro embryo development.

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补充硼酸可通过相关的多能基因和抗氧化基因促进体外生产的小鼠胚胎的发育。

硼酸（BA）是植物、动物和人类的一种重要矿物质，它有助于新陈代谢功能，对生物系统既有积极影响，也有消极影响。本研究旨在探讨在培养基中添加不同浓度的硼酸对小鼠胚胎质量和体外发育潜力的影响。超排卵的 C57Bl6/6j 雌性小鼠在注射人绒毛膜促性腺激素（hCG）18 小时后处死。从输卵管中收集单细胞期胚胎，分成实验组，在补充了 BA+ 的胚胎培养基中培养至囊胚期 96 小时，温度为 37 °C，5% CO2。0、1.62 × 10-1、1.62 × 10-2、1.62 × 10-3 和 1.62 × 10-4 µM BA 的囊胚发育率分别为 51.52%、73.47%、77.36% 和 81.13%。1.62 × 10-3 （p < 0.05）和 1.62 × 10-4 µM BA 组的离体发育率明显高于对照组（p < 0.001）。这些结果表明，低剂量的 BA 对胚胎发育有影响，对体外发育率、胚胎细胞数量、生化参数以及多能基因和抗氧化基因在分子水平上的发育有积极影响。因此，BA 似乎对体外胚胎发育起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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