Xiuyu Wang, Yihan Wang, Qiang Wang, Anita S Chong, Shengbo Cao, Youhui Si
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引用次数: 0
Abstract
Dendritic cells (DCs) play a central role in the initiation of the adaptive immune response. Here, we present a protocol for isolating and transcriptionally profiling antigen-presenting cells (APCs) from the mouse lung and mediastinal lymph nodes (MLNs) following intranasal immunization. We describe steps for preparing single-cell suspensions from the lung and MLN, along with the detection and RNA sequencing (RNA-seq) of antigen-presenting DCs. This protocol offers a broadly applicable approach for identifying variations in DC subpopulations under diverse experimental conditions. For complete details on the use and execution of this protocol, please refer to Youhui et al.1.
树突状细胞(DC)在启动适应性免疫反应中起着核心作用。在这里,我们介绍了一种在鼻内免疫后从小鼠肺部和纵隔淋巴结(MLN)中分离抗原递呈细胞(APCs)并对其进行转录分析的方法。我们描述了从肺部和纵隔淋巴结制备单细胞悬液的步骤,以及抗原呈递 DC 的检测和 RNA 测序(RNA-seq)。该方案提供了一种广泛适用的方法,可用于鉴定不同实验条件下 DC 亚群的变化。有关本方案使用和执行的完整细节,请参阅 Youhui et al.1.