Matthew Waas, Meinusha Govindarajan, Amanda Khoo, Charlotte Zuo, Aastha Aastha, Jilin He, Michael Woolman, Annie Ha, Brian Lin, Thomas Kislinger
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引用次数: 0
Abstract
Deep mass spectrometry-based proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present a protocol for droplet-based one-pot preparation for proteomic samples (DROPPS), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. We describe steps for depositing cellular material, cell lysis, and digesting proteins in the same microliter-droplet well. We anticipate DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations. For complete details on the use and execution of this protocol, please refer to Waas et al.1.
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