Mechanical and suture-holding properties of a UV-cured atelocollagen membrane with varied crosslinked architecture.

Ruya Zhang, Charles Brooker, Laura L E Whitehouse, Neil H Thomson, David Wood, Giuseppe Tronci
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Abstract

The mechanical competence and suturing ability of collagen-based membranes are paramount in guided bone regeneration (GBR) therapy, to ensure damage-free implantation, fixation and space maintenancein vivo. However, contact with the biological medium can induce swelling of collagen molecules, yielding risks of membrane sinking into the bone defect, early loss of barrier function, and irreversibly compromised clinical outcomes. To address these challenges, this study investigates the effect of the crosslinked network architecture on both mechanical and suture-holding properties of a new atelocollagen (AC) membrane. UV-cured networks were obtained via either single functionalisation of AC with 4-vinylbenzyl chloride (4VBC) or sequential functionalisation of AC with both 4VBC and methacrylic anhydride. The wet-state compression modulus (Ec) and swelling ratio (SR) were significantly affected by the UV-cured network architecture, leading up to a three-fold reduction in SR and about two-fold increase inEcin the sequentially functionalised, compared to the single-functionalised, samples. Electron microscopy, dimensional analysis and compression testing revealed the direct impact of the ethanol series dehydration process on membrane microstructure, yielding densification of the freshly synthesised porous samples and a pore-free microstructure with increasedEc. Nanoindentation tests via spherical bead-probe atomic force microscopy (AFM) confirmed an approximately two-fold increase in median (interquartile range (IQR)) elastic modulus in the sequentially functionalised (EAFM= 40 (13) kPa), with respect to single-functionalised (EAFM= 15 (9) kPa), variants. Noteworthy, the single-functionalised, but not the sequentially functionalised, samples displayed higher suture retention strength (SRS = 28 ± 2-35 ± 10 N∙mm-1) in both the dry state and following 1 h in phosphate buffered saline (PBS), compared to Bio-Gide® (SRS: 6 ± 1-14 ± 2 N∙mm-1), while a significant decrease was measured after 24 h in PBS (SRS= 1 ± 1 N∙mm-1). These structure-property relationships confirm the key role played by the molecular architecture of covalently crosslinked collagen, aimed towards long-lasting resorbable membranes for predictable GBR therapy.

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具有不同交联结构的紫外线固化阿特胶原膜的机械性能和缝合固定性能。
在骨引导再生(GBR)疗法中,胶原蛋白膜的机械性能和缝合能力至关重要,可确保体内无损伤植入、固定和空间维持。然而,与生物介质的接触会引起胶原分子膨胀,导致膜沉入骨缺损处、早期丧失屏障功能和不可逆转地影响临床效果等风险。为了应对这些挑战,本研究探讨了交联网络结构对新型阿特劳胶原(AC)膜的机械性能和缝合固定性能的影响。通过 4-乙烯基苄基氯(4VBC)对 AC 进行单一官能化或 4VBC 和甲基丙烯酸酐(MA)对 AC 进行连续官能化,可获得紫外线固化网络。湿态压缩模量(Ec)和膨胀率(SR)受到紫外线固化网络结构的显著影响,与单一官能化的样品相比,顺序官能化的样品的收缩率(SR)降低了三倍,膨胀率(Ec)增加了约两倍。电子显微镜、尺寸分析和压缩测试表明,乙醇系列脱水过程对膜的微观结构产生了直接影响,使新合成的多孔样品变得致密,并形成了无孔的微观结构,同时增加了 Ec。通过球形珠探针原子力显微镜(AFM)进行的纳米压痕测试证实,与单一官能化(EAFM= 15 (9) kPa)相比,顺序官能化(EAFM= 40 (13) kPa)变体的弹性模量中值(四分位间范围)增加了约两倍。值得注意的是,与 Bio-Gide®(SRS:6±1-14±2 N∙mm-1,pSRS= 1±1 N∙mm-1)相比,单一官能化样品在干燥状态下和在磷酸盐缓冲盐水(PBS)中浸泡 1 小时后显示出更高的缝合保持强度(SRS= 28±2─35±10 N∙mm-1),而顺序官能化样品则不然。这些结构-性能关系证实了共价交联胶原的分子结构所起的关键作用,其目的是为可预测的 GBR 治疗提供长效可吸收膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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