Multiplex PCR detection of enteric pathogens in a community-based birth cohort in Ecuador: comparison of xTAG-GPP and TaqMan array card assays.

Stuart Torres Ayala, Lesly Simbana Vivanco, Nikolina Walas, Kelsey J Jesser, Nicolette A Zhou, Christine S Fagnant-Sperati, Hadley R Burroughs, Gwenyth O Lee, Joseph Ns Eisenberg, Gabriel Trueba, Karen Levy, Benjamin F Arnold
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Abstract

We compared the performance of two multiplex platforms, Luminex xTAG Gastrointestinal Pathogen PanelⓇ and TaqMan Array Card, against a panel of 14 enteric pathogen targets in a community-based birth cohort in Ecuador. We found high levels of agreement and similar prevalence estimates across most pathogens.

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厄瓜多尔社区出生队列中肠道病原体的多重 PCR 检测:xTAG-GPP 和 TaqMan 阵列卡检测法的比较。
我们比较了 Luminex xTAG Gastrointestinal Pathogen PanelⓇ 和 TaqMan Array Card 这两种多重平台在厄瓜多尔社区出生队列中针对 14 种肠道病原体目标的性能。我们发现大多数病原体的检测结果具有高度一致性和相似的流行率估计值。
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