Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold

IF 4.7 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Molecular Biology Pub Date : 2024-10-24 DOI:10.1016/j.jmb.2024.168833
Nagendar Goud Badepally, Tales Rocha de Moura, Elżbieta Purta, Eugene F. Baulin, Janusz M. Bujnicki
{"title":"Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold","authors":"Nagendar Goud Badepally,&nbsp;Tales Rocha de Moura,&nbsp;Elżbieta Purta,&nbsp;Eugene F. Baulin,&nbsp;Janusz M. Bujnicki","doi":"10.1016/j.jmb.2024.168833","DOIUrl":null,"url":null,"abstract":"<div><div>Advancements in genome-wide sequence analysis have led to the discovery of numerous novel bacterial non-coding RNAs (ncRNAs). These ncRNAs have been categorized into various RNA families and classes based on their size, structure, function, and evolutionary relationships. One such ncRNA family, <em>raiA,</em> is notably abundant in the bacterial phyla Firmicutes and Actinobacteria and is remarkably well-conserved across many Gram-positive bacteria. In this study, we integrated cryo-electron microscopy single-particle analysis with computational modeling and biochemical techniques to elucidate the structural characteristics of <em>raiA</em> from <em>Clostridium</em> sp. CAG 138. Our findings reveal the globular 3D fold of <em>raiA</em>, providing valuable structural insights. This analysis paves the way for future investigations into the functional properties of <em>raiA</em>, potentially uncovering new regulatory mechanisms in bacterial ncRNAs.</div></div>","PeriodicalId":369,"journal":{"name":"Journal of Molecular Biology","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022283624004625","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Advancements in genome-wide sequence analysis have led to the discovery of numerous novel bacterial non-coding RNAs (ncRNAs). These ncRNAs have been categorized into various RNA families and classes based on their size, structure, function, and evolutionary relationships. One such ncRNA family, raiA, is notably abundant in the bacterial phyla Firmicutes and Actinobacteria and is remarkably well-conserved across many Gram-positive bacteria. In this study, we integrated cryo-electron microscopy single-particle analysis with computational modeling and biochemical techniques to elucidate the structural characteristics of raiA from Clostridium sp. CAG 138. Our findings reveal the globular 3D fold of raiA, providing valuable structural insights. This analysis paves the way for future investigations into the functional properties of raiA, potentially uncovering new regulatory mechanisms in bacterial ncRNAs.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
梭状芽孢杆菌 raiA ncRNA 的低温电子显微镜结构揭示了一种新的 RNA 3D 折叠。
随着全基因组序列分析技术的进步,人们发现了许多新型细菌非编码 RNA(ncRNA)。根据其大小、结构、功能和进化关系,这些 ncRNA 被分为不同的 RNA 家族和类别。其中一个 ncRNA 家族--raiA--在细菌的固氮菌门和放线菌门中非常丰富,而且在许多革兰氏阳性细菌中保存完好。在这项研究中,我们将低温电子显微镜单颗粒分析与计算建模和生物化学技术相结合,阐明了梭状芽孢杆菌 CAG 138 中 raiA 的结构特征。我们的研究结果揭示了 raiA 的球状三维折叠,提供了宝贵的结构见解。这项分析为今后研究 raiA 的功能特性铺平了道路,有可能揭示细菌 ncRNA 的新调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Molecular Biology
Journal of Molecular Biology 生物-生化与分子生物学
CiteScore
11.30
自引率
1.80%
发文量
412
审稿时长
28 days
期刊介绍: Journal of Molecular Biology (JMB) provides high quality, comprehensive and broad coverage in all areas of molecular biology. The journal publishes original scientific research papers that provide mechanistic and functional insights and report a significant advance to the field. The journal encourages the submission of multidisciplinary studies that use complementary experimental and computational approaches to address challenging biological questions. Research areas include but are not limited to: Biomolecular interactions, signaling networks, systems biology; Cell cycle, cell growth, cell differentiation; Cell death, autophagy; Cell signaling and regulation; Chemical biology; Computational biology, in combination with experimental studies; DNA replication, repair, and recombination; Development, regenerative biology, mechanistic and functional studies of stem cells; Epigenetics, chromatin structure and function; Gene expression; Membrane processes, cell surface proteins and cell-cell interactions; Methodological advances, both experimental and theoretical, including databases; Microbiology, virology, and interactions with the host or environment; Microbiota mechanistic and functional studies; Nuclear organization; Post-translational modifications, proteomics; Processing and function of biologically important macromolecules and complexes; Molecular basis of disease; RNA processing, structure and functions of non-coding RNAs, transcription; Sorting, spatiotemporal organization, trafficking; Structural biology; Synthetic biology; Translation, protein folding, chaperones, protein degradation and quality control.
期刊最新文献
Determinants in the HTLV-1 capsid major homology region that are critical for virus particle assembly. Pim1 is Critical in T-cell-independent B-cell Response and MAPK Activation in B Cells. Translation complex profile sequencing allows discrimination of leaky scanning and reinitiation in upstream open reading frame-controlled translation. Chromatin Transcription Elongation - A Structural Perspective. A nanobody toolbox for recognizing distinct epitopes on Cas9.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1