A Conformation-Specific Approach to Native Top-down Mass Spectrometry.

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of the American Society for Mass Spectrometry Pub Date : 2024-12-04 Epub Date: 2024-10-25 DOI:10.1021/jasms.4c00361
Hannah M Britt, Aisha Ben-Younis, Nathanael Page, Konstantinos Thalassinos
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Abstract

Native top-down mass spectrometry is a powerful approach for characterizing proteoforms and has recently been applied to provide similarly powerful insights into protein conformation. Current approaches, however, are limited such that structural insights can only be obtained for the entire conformational landscape in bulk or without any direct conformational measurement. We report a new ion-mobility-enabled method for performing native top-down MS in a conformation-specific manner. Our approach identified conformation-linked differences in backbone dissociation for the model protein calmodulin, which simultaneously informs upon proteoform variations and provides structural insights. We also illustrate that our method can be applied to protein-ligand complexes, either to identify components or to probe ligand-induced structural changes.

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原生自上而下质谱法的构象特异性方法
原生自上而下质谱法是一种表征蛋白质形态的强大方法,最近也被用于提供类似的蛋白质构象洞察力。然而,目前的方法有其局限性,即只能获得整个构象图谱的结构洞察,而无法进行任何直接的构象测量。我们报告了一种以构象特异性方式进行原生自上而下质谱分析的离子迁移率赋能新方法。我们的方法确定了模型蛋白质钙调蛋白骨架解离中与构象相关的差异,这同时告知了蛋白形态的变化并提供了结构见解。我们还说明,我们的方法可应用于蛋白质配体复合物,以确定其成分或探测配体诱导的结构变化。
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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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