Protective effects of electroacupuncture on senile osteoporosis in rats.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Acupuncture in Medicine Pub Date : 2024-12-01 Epub Date: 2024-10-26 DOI:10.1177/09645284241280089

Jun Zhou, Jinling Wang, Mengjian Qu, Qian Wang, Liqiong Wang, Sijia Liu, Jing Liu, Guanghua Sun, Peirui Zhong, Xiarong Huang, Danni Liu, Linwei Yin, Chengqi He

{"title":"Protective effects of electroacupuncture on senile osteoporosis in rats.","authors":"Jun Zhou, Jinling Wang, Mengjian Qu, Qian Wang, Liqiong Wang, Sijia Liu, Jing Liu, Guanghua Sun, Peirui Zhong, Xiarong Huang, Danni Liu, Linwei Yin, Chengqi He","doi":"10.1177/09645284241280089","DOIUrl":null,"url":null,"abstract":"Objectives: The objectives were to explore the protective effects of electroacupuncture (EA) on senile osteoporosis in aged rats and investigate the underlying mechanisms.Methods: This study included aged (24-month-old; n = 16) and young (3-month-old; n = 8) male Sprague-Dawley rats. Aged rats were further randomized 1:1 to an aged control group (Aged; n = 8) and an EA treatment group (EA; n = 8). The 3-month-old rats served as young controls (Young). EA rats received EA at ST36, SP6, GB34 and SP10 bilaterally for 30 min a day, 5 days a week, for 8 weeks.Results: EA significantly increased serum markers of bone formation in Aged rats. There were no significant differences in serum markers of bone resorption between EA and Aged rats. Deterioration of bone mineral density (BMD) and trabecular bone architecture was observed in the Aged group, while EA significantly increased BMD of the left femur and L5 vertebral body in aged rats. Aging-induced deterioration of trabecular bone architecture was partially reversed in EA rats. Runx2 and Osterix mRNA and protein levels were significantly increased and peroxisome proliferator-activated receptor (PPAR)γ was significantly decreased in bone marrow cells in EA compared with Aged groups. The mRNA and protein levels of core constituents of the Wnt/β-catenin signaling pathway (Wnt3a, low-density lipoprotein receptor-related protein (LRP)5 and β-catenin) were significantly increased and Dickkopf 1 was significantly decreased in bone marrow cells in EA compared with Aged groups.Conclusion: EA may prevent bone loss and deterioration in aged rats by promoting osteogenesis via a mechanism that may involve activation of the Wnt/β-catenin signaling pathway. EA may represent a therapeutic option for senile osteoporosis.","PeriodicalId":7257,"journal":{"name":"Acupuncture in Medicine","volume":" ","pages":"334-341"},"PeriodicalIF":2.4000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acupuncture in Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/09645284241280089","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/26 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: The objectives were to explore the protective effects of electroacupuncture (EA) on senile osteoporosis in aged rats and investigate the underlying mechanisms.

Methods: This study included aged (24-month-old; n = 16) and young (3-month-old; n = 8) male Sprague-Dawley rats. Aged rats were further randomized 1:1 to an aged control group (Aged; n = 8) and an EA treatment group (EA; n = 8). The 3-month-old rats served as young controls (Young). EA rats received EA at ST36, SP6, GB34 and SP10 bilaterally for 30 min a day, 5 days a week, for 8 weeks.

Results: EA significantly increased serum markers of bone formation in Aged rats. There were no significant differences in serum markers of bone resorption between EA and Aged rats. Deterioration of bone mineral density (BMD) and trabecular bone architecture was observed in the Aged group, while EA significantly increased BMD of the left femur and L5 vertebral body in aged rats. Aging-induced deterioration of trabecular bone architecture was partially reversed in EA rats. Runx2 and Osterix mRNA and protein levels were significantly increased and peroxisome proliferator-activated receptor (PPAR)γ was significantly decreased in bone marrow cells in EA compared with Aged groups. The mRNA and protein levels of core constituents of the Wnt/β-catenin signaling pathway (Wnt3a, low-density lipoprotein receptor-related protein (LRP)5 and β-catenin) were significantly increased and Dickkopf 1 was significantly decreased in bone marrow cells in EA compared with Aged groups.

Conclusion: EA may prevent bone loss and deterioration in aged rats by promoting osteogenesis via a mechanism that may involve activation of the Wnt/β-catenin signaling pathway. EA may represent a therapeutic option for senile osteoporosis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

电针对大鼠老年性骨质疏松症的保护作用

研究目的目的：探讨电针（EA）对老年骨质疏松症大鼠的保护作用及其机制：研究对象包括老龄（24 月龄，n = 16）和幼龄（3 月龄，n = 8）雄性 Sprague-Dawley 大鼠。老年大鼠按 1:1 随机分为老年对照组（Aged；n = 8）和 EA 治疗组（EA；n = 8）。3个月大的大鼠作为年轻对照组（Young）。EA大鼠在ST36、SP6、GB34和SP10双侧接受EA治疗，每天30分钟，每周5天，共8周：结果：EA 能明显增加老年大鼠骨形成的血清标志物。结果表明：EA 能明显增加老龄大鼠骨形成的血清标志物，而 EA 和老龄大鼠骨吸收的血清标志物无明显差异。老龄组大鼠的骨矿物质密度（BMD）和骨小梁结构出现恶化，而 EA 能明显增加老龄组大鼠左股骨和 L5 椎体的 BMD。EA 大鼠部分逆转了衰老引起的骨小梁结构退化。与衰老组相比，EA 组大鼠骨髓细胞中 Runx2 和 Osterix mRNA 及蛋白水平显著升高，过氧化物酶体增殖激活受体（PPAR）γ 显著降低。与老年组相比，EA组骨髓细胞中Wnt/β-catenin信号通路核心成分（Wnt3a、低密度脂蛋白受体相关蛋白（LRP）5和β-catenin）的mRNA和蛋白水平明显升高，而Dickkopf 1则明显降低：结论：EA可通过一种可能涉及激活Wnt/β-catenin信号通路的机制促进成骨，从而防止老年大鼠骨质流失和退化。EA可能是治疗老年性骨质疏松症的一种选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.