Measurement of the Memory B Cell Response via Antibodies from Activated Cells.

IF 3 Q3 IMMUNOLOGY Antibodies Pub Date : 2024-10-03 DOI:10.3390/antib13040081

Caroline Rockstroh, Katja Hintz, Judith Kannenberg, Christian Jassoy

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Abstract

Background/objectives: The body's immune response to infections and vaccination leads to the formation of memory B cells (MBCs), which protect against future infections. MBCs circulate in the blood, and the strength of the MBC response is measured with different tests. In this study, tests to measure the MBC response were compared.

Methods: An MBC enzyme-linked immunospot assay (MBC-ELISpot), which counts the antibody-releasing cells (MASCs) that arise from memory B cells in vitro, and two versions of an MBC enzyme-linked immunosorbent assay (MBC-ELISA), which measures the concentration of antibodies released by the MASCs, were compared. The lower measurement limit of MBC-ELISpot and ELISA was determined, and it was investigated how the measurement results of individual samples and in a sample of test persons correlate.

Results: Both methods had similar lower limits of detection, and the antibody concentration correlated strongly with the number of MASCs in individual samples. The antibody concentrations measured on a sample correlated with Pearson correlation coefficients of R = 0.83-0.87 with the number of MASCs, and the proportion of antigen-specific antibodies in total IgG correlated with R = 0.74-0.82 with the proportion of antigen-specific MASCs in all antibody-secreting cells.

Conclusions: Since the measurement sensitivity of MBC-ELISA and MBC-ELISpot is similar and the measurement results correlate strongly in a random sample, the tests are interchangeable. The MBC-ELISA has an advantage over the ELISpot in that the antibody measurements can be divided up over time, repeated, and extended. This creates new possibilities for measuring the MBC response.

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通过活化细胞的抗体测量记忆 B 细胞反应

背景/目的：机体对感染和疫苗接种的免疫反应会导致记忆 B 细胞（MBC）的形成，从而保护机体免受未来的感染。记忆性 B 细胞在血液中循环，记忆性 B 细胞反应的强度可通过不同的测试来测量。本研究对测量 MBC 反应的测试方法进行了比较：方法：比较了用于计算体外记忆 B 细胞产生的抗体释放细胞（MASCs）的 MBC 酶联免疫吸附试验（MBC-ELISpot）和用于测量 MASCs 释放的抗体浓度的两种 MBC 酶联免疫吸附试验（MBC-ELISA）。确定了 MBC-ELISpot 和 ELISA 的测量下限，并研究了单个样本的测量结果与受试者样本的测量结果之间的相关性：结果：两种方法的检测下限相似，抗体浓度与单个样本中的 MASC 数量密切相关。样本中测得的抗体浓度与 MASCs 数量的皮尔逊相关系数为 R = 0.83-0.87，抗原特异性抗体在总 IgG 中的比例与抗原特异性 MASCs 在所有抗体分泌细胞中的比例的相关系数为 R = 0.74-0.82：由于MBC-ELISA和MBC-ELISpot的测量灵敏度相似，且在随机样本中的测量结果具有很强的相关性，因此这两种检测方法可以互换。与 ELISpot 相比，MBC-ELISA 的优势在于抗体测量可以分时段进行、重复和扩展。这为测量 MBC 反应提供了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antibodies IMMUNOLOGY-

CiteScore

7.10

自引率

6.40%

发文量

审稿时长

11 weeks

期刊介绍： Antibodies (ISSN 2073-4468), an international, peer-reviewed open access journal which provides an advanced forum for studies related to antibodies and antigens. It publishes reviews, research articles, communications and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided. Electronic files or software regarding the full details of the calculation and experimental procedure - if unable to be published in a normal way - can be deposited as supplementary material. This journal covers all topics related to antibodies and antigens, topics of interest include (but are not limited to): antibody-producing cells (including B cells), antibody structure and function, antibody-antigen interactions, Fc receptors, antibody manufacturing antibody engineering, antibody therapy, immunoassays, antibody diagnosis, tissue antigens, exogenous antigens, endogenous antigens, autoantigens, monoclonal antibodies, natural antibodies, humoral immune responses, immunoregulatory molecules.