Therapeutic effect of berberine against 5-fluorouracil induced ovarian toxicity in rats.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-10-23 DOI:10.1080/10520295.2024.2415005
Selim Demir, Nihal Turkmen Alemdar, Hatice Kucuk, Elif Ayazoglu Demir, Ahmet Menteşe, Yuksel Aliyazıcıoğlu
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Abstract

Berberine (BER) is a naturally occurring alkaloid with a multitude of beneficial effects on human health. Although it is one of the most studied phytochemicals, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. The aim of this study was to investigate the possible protective effect of BER against 5-FU-induced ovotoxicity, focusing on its ability to attenuate oxidative stress, inflammation and apoptosis. The 30 female rats were randomly divided into five groups: Control, BER (2 mg/kg), 5-FU (100 mg/kg), 5-FU+BER (1 mg/kg) and 5-FU+BER (2 mg/kg). The levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and caspase-3 were determined using spectrophotometric methods. In addition, ovarian samples were evaluated histopathologically using hematoxylin&eosin staining method. The MDA, TOS, 8-OHdG, IL-6, TNF-α and caspase-3 levels significantly increased by 5-FU administration. Also, we found that 5-FU significantly decreased TAS, SOD and CAT levels. Treatments with BER significantly attenuated the 5-FU-induced ovarian damage via increasing the antioxidant capacity and reducing the oxidative stress, inflammation and apoptosis in a dose-dependent manner. Moreover, the ovoprotective effect of BER was also confirmed by histopathological evaluation. BER may be evaluated as a potential candidate molecule to reduce 5-FU-induced ovarian toxicity.

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小檗碱对 5-氟尿嘧啶诱导的大鼠卵巢毒性的治疗作用
小檗碱(BER)是一种天然生物碱,对人体健康有多种益处。虽然小檗碱是研究最多的植物化学物质之一,但其对 5-氟尿嘧啶(5-FU)引起的卵巢损伤的治疗效果迄今尚未得到证实。本研究的目的是研究 BER 对 5-FU 引起的卵巢毒性可能具有的保护作用,重点是其减轻氧化应激、炎症和细胞凋亡的能力。30 只雌性大鼠被随机分为五组:对照组、BER(2 毫克/千克)组、5-FU(100 毫克/千克)组、5-FU+BER(1 毫克/千克)组和 5-FU+BER (2 毫克/千克)组。采用分光光度法测定丙二醛(MDA)、总氧化状态(TOS)、总抗氧化状态(TAS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、8-羟基-2'-脱氧鸟苷(8-OHdG)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和Caspase-3的水平。此外,还采用苏木精和伊红染色法对卵巢样本进行了组织病理学评估。结果表明,服用5-FU后,MDA、TOS、8-OHdG、IL-6、TNF-α和caspase-3水平明显升高。我们还发现,5-FU 能明显降低 TAS、SOD 和 CAT 的水平。BER 能提高抗氧化能力,减少氧化应激、炎症和细胞凋亡,从而明显减轻 5-FU 引起的卵巢损伤。此外,组织病理学评估也证实了 BER 的卵巢保护作用。BER可作为一种潜在的候选分子来降低5-FU诱导的卵巢毒性。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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