Therapeutic effect of berberine against 5-fluorouracil induced ovarian toxicity in rats.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-10-23 DOI:10.1080/10520295.2024.2415005
Selim Demir, Nihal Turkmen Alemdar, Hatice Kucuk, Elif Ayazoglu Demir, Ahmet Menteşe, Yuksel Aliyazıcıoğlu
{"title":"Therapeutic effect of berberine against 5-fluorouracil induced ovarian toxicity in rats.","authors":"Selim Demir, Nihal Turkmen Alemdar, Hatice Kucuk, Elif Ayazoglu Demir, Ahmet Menteşe, Yuksel Aliyazıcıoğlu","doi":"10.1080/10520295.2024.2415005","DOIUrl":null,"url":null,"abstract":"<p><p>Berberine (BER) is a naturally occurring alkaloid with a multitude of beneficial effects on human health. Although it is one of the most studied phytochemicals, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. The aim of this study was to investigate the possible protective effect of BER against 5-FU-induced ovotoxicity, focusing on its ability to attenuate oxidative stress, inflammation and apoptosis. The 30 female rats were randomly divided into five groups: Control, BER (2 mg/kg), 5-FU (100 mg/kg), 5-FU+BER (1 mg/kg) and 5-FU+BER (2 mg/kg). The levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and caspase-3 were determined using spectrophotometric methods. In addition, ovarian samples were evaluated histopathologically using hematoxylin&eosin staining method. The MDA, TOS, 8-OHdG, IL-6, TNF-α and caspase-3 levels significantly increased by 5-FU administration. Also, we found that 5-FU significantly decreased TAS, SOD and CAT levels. Treatments with BER significantly attenuated the 5-FU-induced ovarian damage via increasing the antioxidant capacity and reducing the oxidative stress, inflammation and apoptosis in a dose-dependent manner. Moreover, the ovoprotective effect of BER was also confirmed by histopathological evaluation. BER may be evaluated as a potential candidate molecule to reduce 5-FU-induced ovarian toxicity.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":" ","pages":"1-7"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnic & Histochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/10520295.2024.2415005","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Berberine (BER) is a naturally occurring alkaloid with a multitude of beneficial effects on human health. Although it is one of the most studied phytochemicals, its curative effect against ovarian damage caused by 5-fluorouracil (5-FU) has not been demonstrated to date. The aim of this study was to investigate the possible protective effect of BER against 5-FU-induced ovotoxicity, focusing on its ability to attenuate oxidative stress, inflammation and apoptosis. The 30 female rats were randomly divided into five groups: Control, BER (2 mg/kg), 5-FU (100 mg/kg), 5-FU+BER (1 mg/kg) and 5-FU+BER (2 mg/kg). The levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and caspase-3 were determined using spectrophotometric methods. In addition, ovarian samples were evaluated histopathologically using hematoxylin&eosin staining method. The MDA, TOS, 8-OHdG, IL-6, TNF-α and caspase-3 levels significantly increased by 5-FU administration. Also, we found that 5-FU significantly decreased TAS, SOD and CAT levels. Treatments with BER significantly attenuated the 5-FU-induced ovarian damage via increasing the antioxidant capacity and reducing the oxidative stress, inflammation and apoptosis in a dose-dependent manner. Moreover, the ovoprotective effect of BER was also confirmed by histopathological evaluation. BER may be evaluated as a potential candidate molecule to reduce 5-FU-induced ovarian toxicity.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
小檗碱对 5-氟尿嘧啶诱导的大鼠卵巢毒性的治疗作用
小檗碱(BER)是一种天然生物碱,对人体健康有多种益处。虽然小檗碱是研究最多的植物化学物质之一,但其对 5-氟尿嘧啶(5-FU)引起的卵巢损伤的治疗效果迄今尚未得到证实。本研究的目的是研究 BER 对 5-FU 引起的卵巢毒性可能具有的保护作用,重点是其减轻氧化应激、炎症和细胞凋亡的能力。30 只雌性大鼠被随机分为五组:对照组、BER(2 毫克/千克)组、5-FU(100 毫克/千克)组、5-FU+BER(1 毫克/千克)组和 5-FU+BER (2 毫克/千克)组。采用分光光度法测定丙二醛(MDA)、总氧化状态(TOS)、总抗氧化状态(TAS)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、8-羟基-2'-脱氧鸟苷(8-OHdG)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和Caspase-3的水平。此外,还采用苏木精和伊红染色法对卵巢样本进行了组织病理学评估。结果表明,服用5-FU后,MDA、TOS、8-OHdG、IL-6、TNF-α和caspase-3水平明显升高。我们还发现,5-FU 能明显降低 TAS、SOD 和 CAT 的水平。BER 能提高抗氧化能力,减少氧化应激、炎症和细胞凋亡,从而明显减轻 5-FU 引起的卵巢损伤。此外,组织病理学评估也证实了 BER 的卵巢保护作用。BER可作为一种潜在的候选分子来降低5-FU诱导的卵巢毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
期刊最新文献
Grape seed oil attenuates sodium arsenite-induced gastric, hepatic and colonic damage in Wistar rats. Comparison of the protective effects of silymarin and thymoquinone in the focal cerebral ischemia-reperfusion rat model. Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model. Romanowsky staining: history, recent advances and future prospects from a chemistry perspective. Protective effects of chlorogenic acid against cyclophosphamide induced liver injury in mice.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1