snRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-10-25 DOI:10.1186/s12864-024-10913-y
Zhiduan Chi, Qiong Jia, Haoyu Yang, Hongrui Ren, Congli Jin, Jinxin He, Nile Wuri, Ze Sui, Junzhen Zhang, Bayier Mengke, Lixian Zhu, Ge Qiqi, Sarengaowa Aierqing, Ji Wuli, Dong Ai, Ruiwen Fan, Muren Herrid
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Abstract

Background: Mongolian cattle are local breeds in northern China with excellent adaptability to harsh environmental conditions. Adipose tissues play essential roles in tolerance to cold and disease, but the associated cellular and molecular mechanisms are unclear.

Methods: Single-nucleus RNA sequencing (snRNA-seq) was performed on the adipose tissues from the subcutaneous (SAT), greater omentum (OAT) and perirenal (PAT) of 3 healthy cattle. The adipogenic trajectory was analyzed, and the functional roles of gene of interest were verified in vitro.

Results: There were different cell subpopulations in adipose tissues. The lipid-deposition adipocytes identified by the PTGER3 marker exhibited outstanding characteristics in SAT. In PAT and OAT, aldosterone was expressed to provide clues for the differential brown adipocytes. Among the DEGs by comparing OAT with SAT and PAT with OAT, C3 was significantly expressed in most of the cell populations in SAT. G0S2, LIPE, LPIN1, PTGER3 and RGCC took part in the adipogenic trajectory from preadipocyte commitment to mature adipocytes. S100A4 expression affected Ca2+ signaling and the expression of UCP1 ~ 3, FABP4 and PTGER3.

Conclusion: The cell heterogeneity and genes expressed in adipose tissues of Mongolian cattle not only determine the endocrine and energy storage, but contribute to adapt to cold and disease resistance.

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脂肪组织的 snRNA 序列分析揭示了蒙古牛抗寒和抗病的潜在细胞和分子机制。
背景:蒙古牛是中国北方的地方品种,对恶劣的环境条件具有极强的适应性。脂肪组织在耐寒和抗病方面发挥着重要作用,但相关的细胞和分子机制尚不清楚:方法:对 3 头健康牛的皮下(SAT)、大网膜(OAT)和肾周(PAT)脂肪组织进行了单核 RNA 测序(snRNA-seq)。分析了脂肪形成的轨迹,并在体外验证了相关基因的功能作用:结果:脂肪组织中有不同的细胞亚群。结果:脂肪组织中存在不同的细胞亚群。在 SAT 中,通过 PTGER3 标记鉴定的脂质沉积脂肪细胞表现出突出的特征。在 PAT 和 OAT 中,醛固酮的表达为棕色脂肪细胞的差异提供了线索。通过比较 OAT 与 SAT 和 PAT 与 OAT 的 DEGs,发现 C3 在 SAT 的大多数细胞群中都有显著表达。G0S2、LIPE、LPIN1、PTGER3和RGCC参与了前脂肪细胞到成熟脂肪细胞的成脂过程。S100A4 的表达影响了 Ca2+ 信号转导以及 UCP1 ~ 3、FABP4 和 PTGER3 的表达:结论:蒙古牛脂肪组织的细胞异质性和基因表达不仅决定了其内分泌和能量储存,而且有助于适应寒冷和抗病。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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