RNA sequencing comparing centenarian and middle-aged women lymphoblastoid cell lines identifies age-related dysregulated expression of genes encoding selenoproteins, heat shock proteins, CD99, and BID

IF 3.5 4区 医学 Q2 CHEMISTRY, MEDICINAL Drug Development Research Pub Date : 2024-10-24 DOI:10.1002/ddr.70011
Irena Voinsky, Ofir Goldenberg-Bogner, Ifat Israel-Elgali, Hadas Volkov, Monika Puzianowska-Kuźnicka, Noam Shomron, David Gurwitz
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Abstract

Women typically live longer than men, and constitute the majority of centenarians. We applied RNA-sequencing (RNA-seq) of blood-derived lymphoblastoid cell lines (LCLs) from women aged 60-80 years and centenarians (100-105 years), validated the RNA-seq findings by real-time PCR, and additionally measured the differentially expressed genes in LCLs from young women aged 20-35 years. Top RNA-seq genes with differential expression between the age groups included three selenoproteins (GPX1, SELENOW, SELENOH) and three heat shock proteins (HSPA6, HSPA1A, HSPA1B), with the highest expression in LCLs from young women, indicating that young women are better protected from oxidative stress. The expression of two additional genes, BID encoding BH3-interacting domain death agonist and CD99 encoding CD99 antigen, showed unique age dependence, with similar expression levels in young and centenarian women while exhibiting higher and lower expression levels, respectively, in LCLs from women aged 60-80 years compared with the two other age groups. This age-related differential expression of BID and CD99 suggests elevated inflammation susceptibility in middle-aged women compared with either young or centenarian women. Our findings, once validated with human peripheral blood mononuclear cells and further cell types, may lead to novel healthy aging diagnostics and therapeutics.

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通过对百岁老人和中年女性淋巴母细胞系进行 RNA 测序比较,发现与年龄有关的硒蛋白、热休克蛋白、CD99 和 BID 编码基因表达失调。
女性的寿命通常比男性长,而且在百岁老人中占大多数。我们对来自 60-80 岁女性和百岁老人(100-105 岁)的血源性淋巴母细胞系(LCLs)进行了 RNA 序列分析(RNA-seq),并通过实时 PCR 验证了 RNA-seq 结果,此外还测定了来自 20-35 岁年轻女性的 LCLs 中的差异表达基因。不同年龄组之间存在差异表达的顶级RNA-seq基因包括三种硒蛋白(GPX1、SELENOW、SELENOH)和三种热休克蛋白(HSPA6、HSPA1A、HSPA1B),其中年轻女性的LCL中表达量最高,这表明年轻女性在氧化应激方面的保护能力更强。另外两个基因,即编码 BH3-相互作用结构域死亡激动剂的 BID 和编码 CD99 抗原的 CD99 的表达表现出独特的年龄依赖性,在年轻女性和百岁女性中的表达水平相似,而在 60-80 岁女性的 LCL 中的表达水平则分别高于和低于其他两个年龄组。与年龄相关的 BID 和 CD99 表达差异表明,与年轻女性或百岁女性相比,中年女性对炎症的易感性更高。我们的发现一旦在人类外周血单核细胞和其他细胞类型中得到验证,可能会带来新的健康老龄化诊断和治疗方法。
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来源期刊
CiteScore
6.40
自引率
2.60%
发文量
104
审稿时长
6-12 weeks
期刊介绍: Drug Development Research focuses on research topics related to the discovery and development of new therapeutic entities. The journal publishes original research articles on medicinal chemistry, pharmacology, biotechnology and biopharmaceuticals, toxicology, and drug delivery, formulation, and pharmacokinetics. The journal welcomes manuscripts on new compounds and technologies in all areas focused on human therapeutics, as well as global management, health care policy, and regulatory issues involving the drug discovery and development process. In addition to full-length articles, Drug Development Research publishes Brief Reports on important and timely new research findings, as well as in-depth review articles. The journal also features periodic special thematic issues devoted to specific compound classes, new technologies, and broad aspects of drug discovery and development.
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