Associations Between Developmental Stability, Canalization, and Phenotypic Plasticity in Response to Heterogeneous Experience

Abstract

The processes of developmental stability, canalization, and phenotypic plasticity have ecological and evolutionary significance, and been studied extensively, but mostly separately and thus the relationships between them are not straightforward. Our objective was to better integrate these processes in the context of temporally heterogeneous environments. We did this by investigating the effects of early experience with temporal heterogeneity in water availability on associations between developmental stability, canalization, and phenotypic plasticity. We subjected eight plant species to a first round of alternating inundation and drought vs. constantly moderate water treatments (heterogeneous experience) and a second round of water conditions (to test plasticity). We measured fluctuating asymmetry (FA) in leaf size, intra- and inter-individual variation (CV_intra and CV_inter), and plasticity (PI) in traits and analyzed correlations between these variables across all species. Results showed little correlations between FA, CV_intra and PI, several positive correlations between FA and CV_inter in more stressful conditions, especially in as well as positive correlations between CV_inter and PI initially and negative correlations between them later. These suggested the complexity of these relationships, which can depend on whether plasticity occurs. Greater inter-individual variation will more likely cooperate with plasticity before or during plastic response, whereas higher canalization may reflect phenotypic convergence. Both higher FA and CV_intra can reflect faster growth, while CV_intra may also reflect plant growth stage, and the two mechanisms should cooperate in response to environmental challenges. The complexity of these relationships suggests plants deal with environmental variation in elaborate and integrative ways which can be affected by many factors.