New insight into acid-resistant enzymes from natural mutations of Escherichia coli Nissle 1917

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2024-10-20 DOI:10.1016/j.enzmictec.2024.110526

Chengfeng Xue, Wan-Wen Ting, Jiun-Jang Juo, I-Son Ng

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Abstract

The probiotic Escherichia coli Nissle 1917 (EcN), known for its superior acid resistance (AR), serves as a promising chassis for live therapeutics due to the effective colonization capabilities. However, the enzymatic activity regarding AR in EcN remains poorly understood. First, we investigated the AR systems of EcN by measuring cell growth under acidic stress and exploring the relationship of mutations to their corresponding enzymatic activities. As a result, the catalytic activity of inducible decarboxylases of GadB, AdiA and CadA, responsible for metabolizing glutamate, arginine, and lysine, exhibited an average 2-fold increase in EcN compared to the reference strain MG1655. Furthermore, we discovered that the glutamate-dependent AR2 system in EcN was meticulously regulated by specific regulons such as GadW. This study not only revealed the physiology of EcN under acidic conditions, but also highlighted that the mutated core enzymes in the AR system of EcN exhibit improved activities.

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从大肠杆菌 Nissle 1917 的天然突变中了解耐酸酶的新发现。

益生菌大肠埃希氏菌 Nissle 1917（EcN）以其卓越的耐酸性（AR）而闻名，由于其有效的定植能力，它是一种很有前景的活体疗法底盘。然而，人们对 EcN 中 AR 的酶活性仍然知之甚少。首先，我们通过测量酸性胁迫下的细胞生长来研究 EcN 的 AR 系统，并探索突变与其相应酶活性的关系。结果发现，与参考菌株 MG1655 相比，EcN 中负责谷氨酸、精氨酸和赖氨酸代谢的诱导性脱羧酶 GadB、AdiA 和 CadA 的催化活性平均增加了 2 倍。此外，我们还发现 EcN 中依赖谷氨酸的 AR2 系统受到 GadW 等特定调控子的严格调控。这项研究不仅揭示了 EcN 在酸性条件下的生理机理，还突出显示了 EcN AR 系统中突变的核心酶表现出更高的活性。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.