Comparative Transcriptome Analysis of Cold Tolerance Mechanism in Honeybees (Apis mellifera sinisxinyuan).

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY Insects Pub Date : 2024-10-11 DOI:10.3390/insects15100790
Jinqiong Shan, Ruiyi Cheng, Tuohudasheng Magaoya, Yujie Duan, Chao Chen
{"title":"Comparative Transcriptome Analysis of Cold Tolerance Mechanism in Honeybees (<i>Apis mellifera sinisxinyuan</i>).","authors":"Jinqiong Shan, Ruiyi Cheng, Tuohudasheng Magaoya, Yujie Duan, Chao Chen","doi":"10.3390/insects15100790","DOIUrl":null,"url":null,"abstract":"<p><p>Honeybees are important pollinators worldwide that are closely related to agricultural production and ecological balance. The biological activities and geographical distribution of honeybees are strongly influenced by temperature. However, there is not much research on the cold tolerance of honeybees. The <i>Apis mellifera sinisxinyuan</i>, a kind of western honeybee, exhibits strong cold hardiness. Here, we determined that short-term temperature treatment could regulate the honeybee's cold tolerance ability by measuring the supercooling point of <i>A. m. sinisxinyuan</i> treated with different temperatures. Transcriptome data were analyzed between the treated and untreated honeybees. A total of 189 differentially expressed genes were identified. Among them, <i>Abra</i>, <i>Pla1</i>, <i>rGC</i>, <i>Hr38</i>, and <i>Maf</i> were differentially expressed in all comparisons. GO and KEGG analysis showed that the DEGs were enriched in molecular functions related to disease, signal transduction, metabolism, and the endocrine system's function. The main components involved were ribosomes, nucleosomes, proteases, and phosphokinases, among others. This study explored the formation and regulation mechanism of cold tolerance in honeybees, not only providing a theoretical basis for cultivating honeybees with excellent traits but also promoting research and practice on insect stress tolerance.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508713/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insects","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/insects15100790","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Honeybees are important pollinators worldwide that are closely related to agricultural production and ecological balance. The biological activities and geographical distribution of honeybees are strongly influenced by temperature. However, there is not much research on the cold tolerance of honeybees. The Apis mellifera sinisxinyuan, a kind of western honeybee, exhibits strong cold hardiness. Here, we determined that short-term temperature treatment could regulate the honeybee's cold tolerance ability by measuring the supercooling point of A. m. sinisxinyuan treated with different temperatures. Transcriptome data were analyzed between the treated and untreated honeybees. A total of 189 differentially expressed genes were identified. Among them, Abra, Pla1, rGC, Hr38, and Maf were differentially expressed in all comparisons. GO and KEGG analysis showed that the DEGs were enriched in molecular functions related to disease, signal transduction, metabolism, and the endocrine system's function. The main components involved were ribosomes, nucleosomes, proteases, and phosphokinases, among others. This study explored the formation and regulation mechanism of cold tolerance in honeybees, not only providing a theoretical basis for cultivating honeybees with excellent traits but also promoting research and practice on insect stress tolerance.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
蜜蜂耐寒机制的转录组比较分析
蜜蜂是全球重要的授粉动物,与农业生产和生态平衡密切相关。蜜蜂的生物活动和地理分布受温度的影响很大。然而,有关蜜蜂耐寒性的研究并不多。西新元蜜蜂(Apis mellifera sinisxinyuan)是西方蜜蜂的一种,具有很强的耐寒性。在此,我们通过测量经不同温度处理的西新元蜂的过冷却点,确定短期温度处理可调节蜜蜂的耐寒能力。分析了处理蜜蜂和未处理蜜蜂的转录组数据。共鉴定出 189 个差异表达基因。其中,Abra、Pla1、rGC、Hr38和Maf在所有比较中均有差异表达。GO和KEGG分析表明,DEGs富集在与疾病、信号转导、新陈代谢和内分泌系统功能有关的分子功能中。涉及的主要成分包括核糖体、核小体、蛋白酶和磷酸激酶等。该研究探索了蜜蜂耐寒性的形成和调控机制,不仅为培育优良性状的蜜蜂提供了理论依据,也促进了昆虫抗逆性的研究和实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Insects
Insects Agricultural and Biological Sciences-Insect Science
CiteScore
5.10
自引率
10.00%
发文量
1013
审稿时长
21.77 days
期刊介绍: Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
期刊最新文献
Impact of Climate Change on Peach Fruit Moth Phenology: A Regional Perspective from China. Courtship Behavior of Adult Spodoptera frugiperda (Lepidoptera: Noctuidae) Observed Using Track 3D Trajectory Tracking. Hand Warmer-Induced Hypoxia Accelerates Pest Control in Hermetic Storage. Major Insect Pests of Sweet Potatoes in Brazil and the United States, with Information on Crop Production and Regulatory Pest Management. Modelling Blow Fly (Diptera: Calliphoridae) Spatiotemporal Species Richness and Total Abundance Across Land-Use Types.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1