The Cumulative Variations of Respiratory Syncytial Virus Fusion Protein (F) in Ten Consecutive Years in China.

IF 3.4 Q2 INFECTIOUS DISEASES Infectious Disease Reports Pub Date : 2024-10-17 DOI:10.3390/idr16050081

Fengjie Wang, Mingli Jiang, Zhenzhi Han, Yanpeng Xu, Yu Sun, Runan Zhu, Dongmei Chen, Qi Guo, Yutong Zhou, Yao Yao, Ling Cao, Dong Qu, Muya Li, Linqing Zhao

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Abstract

Background: Variations in the fusion (F) protein of respiratory syncytial virus (RSV) with main antigenic sites I-V and Ø may affect the development of RSV vaccines and therapies.

Methods: In the study, 30 respiratory specimens positive for RSV were randomly selected from children with acute lower respiratory infections (ALRI) in Beijing every year from 2012 to 2021 for F gene sequencing. Then, 300 F gene sequences and 508 uploaded to GenBank from China were subjected to phylogenetic analysis.

Results: The results indicated the nucleotide identities were 95.4-100% among 446 sequences of RSV A, and 96.3-100% among 362 of RSV B. The most common variant loci were N80K (100.00%) and R213S (97.76%) for site Ø, and V384I/T (98.43%) for site I among sequences of RSV A, and M152I (100.00%), I185V (100.00%), and L172Q/H (94.48%) for site V, and R202Q (99.45%) for site Ø among sequences of RSV B. N276S appears in 95.29% sequences of RSV A, while S276N and N262 I/S appear in 1.38% and 0.55% sequences of RSV B, respectively. No variation was found in all sequences at the binding sites of 14N4 and motavizumab.

Conclusions: There were cumulative variations of the RSV F gene, especially at some binding sites of antigenic sites.

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中国呼吸道合胞病毒融合蛋白（F）连续十年的累积变化。

背景：呼吸道合胞病毒（RSV）主要抗原位点I-V和Ø的融合蛋白（F）的变异可能会影响RSV疫苗和疗法的开发：该研究从2012年至2021年每年从北京市急性下呼吸道感染（ALRI）患儿中随机抽取30份RSV阳性呼吸道标本进行F基因测序。然后，对中国300个F基因序列和508个上传到GenBank的F基因序列进行系统发育分析：结果表明，在 RSV A 的 446 条序列中，核苷酸同一性为 95.4%-100%；在 RSV B 的 362 条序列中，核苷酸同一性为 96.3%-100%；在 RSV A 的序列中，最常见的变异位点是位点Ø的 N80K（100.00%）和 R213S（97.76%）以及位点Ⅰ的 V384I/T（98.43%）；在 RSV B 的序列中，最常见的变异位点是位点Ⅰ的 M152I（100.在 RSV B 的序列中，位点 V 为 M152I（100.00%）、I185V（100.00%）和 L172Q/H（94.48%），位点 Ø 为 R202Q（99.45%）。N276S 出现在 95.29% 的 RSV A 序列中，而 S276N 和 N262 I/S 分别出现在 1.38% 和 0.55% 的 RSV B 序列中。在 14N4 和莫他珠单抗结合位点的所有序列中均未发现变异：结论：RSV F 基因存在累积变异，尤其是在抗原位点的某些结合位点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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