A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells.

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-10-16 DOI:10.3390/jfb15100309
Jesús Alejandro Torres-Betancourt, Rene Hernández-Delgadillo, Juan Valerio Cauich-Rodríguez, Diego Adrián Oliva-Rico, Juan Manuel Solis-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Nayely Pineda-Aguilar, Samantha Flores-Treviño, Irene Meester, Sergio Eduardo Nakagoshi-Cepeda, Katiushka Arevalo-Niño, María Argelia Akemi Nakagoshi-Cepeda, Claudio Cabral-Romero
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Abstract

Electrospun membranes (EMs) have a wide range of applications, including use as local delivery systems. In this study, we manufactured a polyurethane Tecoflex™ EM loaded with bismuth-based lipophilic nanoparticles (Tecoflex™ EMs-BisBAL NPs). The physicochemical and mechanical characteristics, along with the antitumor and bactericidal effects, were evaluated using a breast cancer cell line and methicillin-susceptible and resistant Staphylococcus aureus (MRSA). Drug-free Tecoflex™ EMs and Tecoflex™ EMs-BisBAL NPs had similar fiber diameters of 4.65 ± 1.42 µm and 3.95 ± 1.32 µm, respectively. Drug-free Tecoflex™ EMs did not negatively impact a human fibroblast culture, indicating that the vehicle is biocompatible. Tecoflex™ EMs-BisBAL NPs increased 94% more in size than drug-free Tecoflex™ EMs, indicating that the BisBAL NPs enhanced hydration capacity. Tecoflex™ EMs-BisBAL NPs were highly bactericidal against both methicillin-susceptible S. aureus and MRSA clinical isolates, inhibiting their growth by 93.11% and 61.70%, respectively. Additionally, Tecoflex™ EMs-BisBAL NPs decreased the viability of MCF-7 tumor cells by 86% after 24 h exposure and 70.1% within 15 min. Regarding the mechanism of action of Tecoflex™ EMs-BisBAL NPs, it appears to disrupt the tumor cell membrane. In conclusion, Tecoflex™ EMs-BisBAL NPs constitute an innovative low-cost drug delivery system for human breast cancer and postoperative wound infections.

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含亲脂性铋纳米颗粒(BisBAL NPs)的聚氨酯电纺膜:增殖、杀菌和抗肿瘤特性,以及对 MRSA 和人类乳腺癌细胞的影响。
电纺丝膜(EM)具有广泛的应用,包括用作局部给药系统。在这项研究中,我们制造了一种聚氨酯 Tecoflex™ EM,其中装载了亲脂性铋纳米颗粒(Tecoflex™ EMs-BisBAL NPs)。使用乳腺癌细胞系和甲氧西林敏感和耐药金黄色葡萄球菌(MRSA)对其理化和机械特性以及抗肿瘤和杀菌效果进行了评估。无药 Tecoflex™ EMs 和 Tecoflex™ EMs-BisBAL NPs 的纤维直径相似,分别为 4.65 ± 1.42 µm 和 3.95 ± 1.32 µm。不含药物的 Tecoflex™ EMs 不会对人类成纤维细胞的培养产生负面影响,这表明载体具有生物相容性。与不含药物的 Tecoflex™ EMs 相比,Tecoflex™ EMs-BisBAL NPs 的尺寸增加了 94%,这表明 BisBAL NPs 增强了水合能力。Tecoflex™ EMs-BisBAL NPs 对甲氧西林敏感的金黄色葡萄球菌和 MRSA 临床分离菌有很强的杀菌作用,对其生长的抑制率分别为 93.11% 和 61.70%。此外,暴露 24 小时后,Tecoflex™ EMs-BisBAL NPs 可使 MCF-7 肿瘤细胞的存活率降低 86%,15 分钟内降低 70.1%。关于 Tecoflex™ EMs-BisBAL NPs 的作用机制,它似乎能破坏肿瘤细胞膜。总之,Tecoflex™ EMs-BisBAL NPs 是一种创新的低成本给药系统,可用于治疗人类乳腺癌和术后伤口感染。
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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
期刊最新文献
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