Isolation of Bacterial Endophytes Associated with Cinchona ledgeriana Moens. and Their Potential in Plant-growth Promotion, Antifungal and Quinoline Alkaloids Production.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-10-25 DOI:10.2323/jgam.2024.09.002
Fauzi Akhbar Anugrah, I Nyoman Pugeg Aryantha, Rahmi Masita, Siti Zubaidah, Nur Izzati Mohd Noh
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Abstract

For centuries, quinoline alkaloids from the tree bark of Cinchona ledgeriana (C. ledgeriana) have been used in the treatment of malaria. However, unsustainable harvesting and poor growth conditions greatly limit its use as raw materials. Since plant endophytes are known to contribute to the physiology of the host and its metabolism for survival, this study showed the potential of endophytes isolated from C. ledgeriana roots in promoting the germination of Catharathus roseus (C. roseus) seedlings and the biosynthesis of quinoline alkaloid. In this present study, we found that the Enterobacteriaceae family comprised the majority of the bacterial community, with Klebsiella pneumoniae being the most abundant species at the C. ledgeriana roots. Characterization of culturable bacterial endophytes from the C. ledgeriana roots showed that all the isolates displayed plant growth-promoting factors and antifungal activities. Interestingly, chromatographic analyses led to the identification of the quinoline alkaloids producing Achromobacter xylosoxidans (A. xylosoxidans) A1. Moreover, the co-cultures of A. xylosoxidans A1, Cytobacillus solani (C. solani) A3, and Klebsiella aerogenes A6 increased the fresh and dry weight of the C. roseus seedlings. These results suggest that these bacterial endophytes may enhance quinine and quinidine production as well as the growth of the plant host.

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Cinchona ledgeriana Moens.的内生细菌分离及其在促进植物生长、抗真菌和生产喹啉生物碱方面的潜力。
几个世纪以来,金鸡纳树(Cinchona ledgeriana)树皮中的喹啉生物碱一直被用于治疗疟疾。然而,不可持续的采伐和恶劣的生长条件极大地限制了其作为原材料的使用。众所周知,植物内生菌有助于宿主的生理机能和新陈代谢,从而促进宿主的生存。本研究表明,从 C. ledgeriana 根部分离的内生菌具有促进 Catharathus roseus(C. roseus)幼苗发芽和喹啉生物碱生物合成的潜力。在本研究中,我们发现肠杆菌科细菌占细菌群落的大多数,其中肺炎克雷伯氏菌是 C. ledgeriana 根部最多的菌种。对 C. ledgeriana 根部可培养的细菌内生菌的特性分析表明,所有分离菌株都具有促进植物生长的因子和抗真菌活性。有趣的是,通过色谱分析确定了产生喹啉生物碱的木质氧化牛膝杆菌(A. xylosoxidans)A1。此外,A. xylosoxidans A1、Cytobacillus solani (C. solani) A3 和 Klebsiella aerogenes A6 的共培养增加了蔷薇幼苗的鲜重和干重。这些结果表明,这些细菌内生菌可提高奎宁和奎尼丁的产量,并促进植物宿主的生长。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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