{"title":"Characterization of a High-Affinity Copper Transporter <i>CTR1a</i> in the White-Nose Syndrome Causing Fungal Pathogen <i>Pseudogymnoascus destructans</i>.","authors":"Saika Anne, Alyssa D Friudenberg, Ryan L Peterson","doi":"10.3390/jof10100729","DOIUrl":null,"url":null,"abstract":"<p><p>Copper is an essential micronutrient and the ability to scavenge tightly bound or trace levels of copper ions at the host-pathogen interface is vital for fungal proliferation in animal hosts. Recent studies suggest that trace metal ion acquisition is critical for the establishment and propagation of <i>Pseudogymnoascus destructans</i>, the fungal pathogen responsible for white-nose syndrome (WNS), on their bat host. However, little is known about these metal acquisition pathways in <i>P. destructans.</i> In this study, we report the characterization of the <i>P. destructans</i> high-affinity copper transporter VC83_00191 (<i>Pd</i>CTR1a), which is implicated as a virulence factor associated with the WNS disease state. Using <i>Saccharomyces cerevisiae</i> as a recombinant expression host, we find that <i>Pd</i>CTR1a can efficiently traffic Cu ions into the yeast cytoplasm. Complementary studies in the native <i>P. destructans</i> fungus provide evidence that <i>Pd</i>CTR1a transcripts and protein levels are dictated by Cu-bioavailability in the growth media. Our study demonstrates that <i>Pd</i>CTR1a is a functional high-affinity copper transporter and is relevant to Cu homeostasis pathways in <i>P. destructans.</i></p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509074/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fungi","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/jof10100729","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Copper is an essential micronutrient and the ability to scavenge tightly bound or trace levels of copper ions at the host-pathogen interface is vital for fungal proliferation in animal hosts. Recent studies suggest that trace metal ion acquisition is critical for the establishment and propagation of Pseudogymnoascus destructans, the fungal pathogen responsible for white-nose syndrome (WNS), on their bat host. However, little is known about these metal acquisition pathways in P. destructans. In this study, we report the characterization of the P. destructans high-affinity copper transporter VC83_00191 (PdCTR1a), which is implicated as a virulence factor associated with the WNS disease state. Using Saccharomyces cerevisiae as a recombinant expression host, we find that PdCTR1a can efficiently traffic Cu ions into the yeast cytoplasm. Complementary studies in the native P. destructans fungus provide evidence that PdCTR1a transcripts and protein levels are dictated by Cu-bioavailability in the growth media. Our study demonstrates that PdCTR1a is a functional high-affinity copper transporter and is relevant to Cu homeostasis pathways in P. destructans.
期刊介绍:
Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.