Characterization of a High-Affinity Copper Transporter CTR1a in the White-Nose Syndrome Causing Fungal Pathogen Pseudogymnoascus destructans.

IF 4.2 2区 生物学 Q2 MICROBIOLOGY Journal of Fungi Pub Date : 2024-10-21 DOI:10.3390/jof10100729
Saika Anne, Alyssa D Friudenberg, Ryan L Peterson
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Abstract

Copper is an essential micronutrient and the ability to scavenge tightly bound or trace levels of copper ions at the host-pathogen interface is vital for fungal proliferation in animal hosts. Recent studies suggest that trace metal ion acquisition is critical for the establishment and propagation of Pseudogymnoascus destructans, the fungal pathogen responsible for white-nose syndrome (WNS), on their bat host. However, little is known about these metal acquisition pathways in P. destructans. In this study, we report the characterization of the P. destructans high-affinity copper transporter VC83_00191 (PdCTR1a), which is implicated as a virulence factor associated with the WNS disease state. Using Saccharomyces cerevisiae as a recombinant expression host, we find that PdCTR1a can efficiently traffic Cu ions into the yeast cytoplasm. Complementary studies in the native P. destructans fungus provide evidence that PdCTR1a transcripts and protein levels are dictated by Cu-bioavailability in the growth media. Our study demonstrates that PdCTR1a is a functional high-affinity copper transporter and is relevant to Cu homeostasis pathways in P. destructans.

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白鼻综合征致病真菌假革囊菌中高亲和性铜转运体 CTR1a 的特征。
铜是一种必需的微量营养元素,在宿主-病原体界面清除紧密结合或痕量水平的铜离子的能力对于真菌在动物宿主中的繁殖至关重要。最近的研究表明,痕量金属离子的获取对于导致白鼻综合症(WNS)的真菌病原体--破坏性假丝酵母菌(Pseudogymnoascus destructans)在蝙蝠宿主上的建立和繁殖至关重要。然而,人们对毁灭假丝酵母的这些金属获取途径知之甚少。在本研究中,我们报告了毁坏真菌高亲和性铜转运体 VC83_00191 (PdCTR1a)的特征,它被认为是与 WNS 疾病状态相关的毒力因子。利用酿酒酵母作为重组表达宿主,我们发现 PdCTR1a 能有效地将铜离子转运到酵母细胞质中。在原生毁灭酵母菌中进行的补充研究证明,PdCTR1a 的转录本和蛋白质水平受生长介质中铜生物可用性的影响。我们的研究证明,PdCTR1a 是一种功能性高亲和性铜转运体,与去壳酵母中的铜平衡途径有关。
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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
期刊最新文献
Characterization of a High-Affinity Copper Transporter CTR1a in the White-Nose Syndrome Causing Fungal Pathogen Pseudogymnoascus destructans. Diversity and Antifungal Susceptibilities of Yeasts from Mangroves in Hong Kong, China-A One Health Aspect. Recent Advances in Diagnostic Approaches for Mucormycosis. Exogenous Nitric Oxide Induces Pathogenicity of Alternaria alternata on Huangguan Pear Fruit by Regulating Reactive Oxygen Species Metabolism and Cell Wall Modification. Three New Species of Mytilinidioid Fungi (Dothideomycetes, Ascomycota) from Mexico.
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