Hyaluronic acid/chitin thermosensitive hydrogel loaded with TGF-β1 promotes meniscus repair in rabbit meniscus full-thickness tear model.

IF 2.8 3区医学 Q1 ORTHOPEDICS Journal of Orthopaedic Surgery and Research Pub Date : 2024-10-23 DOI:10.1186/s13018-024-05144-6

Ze Wang, Wei Huang, Shengyang Jin, Fei Gao, Tingfang Sun, Yu He, Xulin Jiang, Hong Wang

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Abstract

Repair of the damaged meniscus is a scientific challenge owing to the poor self-healing potential of the white area of the meniscus. Tissue engineering provides a new method for the repair of meniscus injuries. In this study, we explored the superiority of 2% hyaluronic acid chitin hydrogel in temperature sensitivity, in vitro degradation, biocompatibility, cell adhesion, and other biological characteristics, and investigated the advantages of hyaluronic acid (HA) and Transforming Growth Factor β1 (TGF-β1) in promoting cell proliferation and a matrix formation phenotype. The hydrogel loaded with HA and TGF-β1 promoted cell proliferation. The HA + TGF-β1 mixed group showed the highest glycosaminoglycan (GAG) content and promoted cell migration. Hydroxypropyl chitin (HPCH), HA, and TGF-β1 were combined to form a composite hydrogel with a concentration of 2% after physical cross-linking, and this was injected into a rabbit model of a meniscus full-thickness tear. After 12 weeks of implantation, the TGF-β1 + HA/HPCH composite hydrogel was significantly better than HPCH, HA/HPCH, TGF-β1 + HPCH, and the control group in promoting meniscus repair. In addition, the new meniscus tissue of the TGF-β1 + HA/HPCH composite hydrogel had a tissue structure and biochemical content similar to that of the normal meniscus tissue.

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含 TGF-β1 的透明质酸/甲壳素热敏水凝胶促进兔半月板全厚撕裂模型的半月板修复

由于半月板白色区域的自愈能力较差，修复受损的半月板是一项科学挑战。组织工程学为修复半月板损伤提供了一种新方法。在这项研究中，我们探讨了 2% 透明质酸甲壳素水凝胶在温度敏感性、体外降解、生物相容性、细胞粘附性等生物学特性方面的优越性，并研究了透明质酸（HA）和转化生长因子β1（TGF-β1）在促进细胞增殖和基质形成表型方面的优势。含有 HA 和 TGF-β1 的水凝胶可促进细胞增殖。HA + TGF-β1 混合组的糖胺聚糖（GAG）含量最高，并能促进细胞迁移。将羟丙基甲壳素（HPCH）、HA 和 TGF-β1 经过物理交联后形成浓度为 2% 的复合水凝胶，并将其注射到半月板全厚撕裂的兔子模型中。植入 12 周后，在促进半月板修复方面，TGF-β1 + HA/HPCH 复合水凝胶明显优于 HPCH、HA/HPCH、TGF-β1 + HPCH 和对照组。此外，TGF-β1 + HA/HPCH 复合水凝胶的新半月板组织的组织结构和生化成分与正常半月板组织相似。

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来源期刊

Journal of Orthopaedic Surgery and Research ORTHOPEDICS-

CiteScore

4.10

自引率

7.70%

发文量

494

审稿时长

>12 weeks

期刊介绍： Journal of Orthopaedic Surgery and Research is an open access journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal issues. Orthopaedic research is conducted at clinical and basic science levels. With the advancement of new technologies and the increasing expectation and demand from doctors and patients, we are witnessing an enormous growth in clinical orthopaedic research, particularly in the fields of traumatology, spinal surgery, joint replacement, sports medicine, musculoskeletal tumour management, hand microsurgery, foot and ankle surgery, paediatric orthopaedic, and orthopaedic rehabilitation. The involvement of basic science ranges from molecular, cellular, structural and functional perspectives to tissue engineering, gait analysis, automation and robotic surgery. Implant and biomaterial designs are new disciplines that complement clinical applications. JOSR encourages the publication of multidisciplinary research with collaboration amongst clinicians and scientists from different disciplines, which will be the trend in the coming decades.