Immunomodulatory effect of Tityus sp. in mononuclear cells extracted from the blood of rheumatoid arthritis patients.

IF 1.8 3区 医学 Q4 TOXICOLOGY Journal of Venomous Animals and Toxins Including Tropical Diseases Pub Date : 2024-09-16 eCollection Date: 2024-01-01 DOI:10.1590/1678-9199-JVATITD-2023-0064
Cindy Gabriela Rivera Tobar, Yisel Del Mar Morales Urmendiz, Marcela Alejandra Vallejo, Diego Felipe Manquillo, Victoria Eugenia Niño Castaño, Ana Isabel Ospina Caicedo, Leydy Lorena Mendoza Tobar, Jimmy Alexander Guerrero Vargas, Rosa Amalia Dueñas Cuellar
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Abstract

Background: Pathophysiological mechanisms of rheumatoid arthritis arise because of a proinflammatory environment, generated by the interaction of autoreactive lymphocytes and proinflammatory mediators. Current strategies to mitigate the progression of the disease produce adverse effects, so there is a need for new therapeutic strategies and molecular targets to treat this disease. In this context, evidence suggests that scorpion venoms could modulate the immune response and some important cellular mechanisms of pharmacological interest. To evaluate the immunomodulatory effect of the venom of Tityus sp. (a possible new species close to Tityus metuendus) peripheral blood mononuclear cells of women diagnosed with RA were compared to cells of a control group.

Methods: A case-control study was conducted with a sample of 10 women with a confirmed diagnosis of RA and controls matched by sex and age. The cytotoxicity of the venom was evaluated to find sublethal concentrations of the venom, and subsequently, their immunomodulatory capacity in terms of percentage of proliferation, cell activation, and cytokines production.

Results: the venom of Tityus sp. produced a decrease in the percentage of proliferation in the CD3+, CD3+CD4+, and CD3+CD8+ cell subpopulations of RA patients and healthy controls, at concentrations of 252 and 126 µg/mL. However, the venom did not induce significant differences in the percentage of cell activation markers. The venom caused a decrease in IL-10 at a concentration of 252 µg/mL compared to untreated cells from patients and controls. The remaining cytokines did not show significant differences.

Conclusion: the venom of Tityus sp. is a potential source of molecules with immunomodulatory ability in CD4 and CD8 T lymphocytes. This result directs venom characterization studies to identify pharmacological targets with immunomodulatory capacity in T lymphocytes to enhance research in the treatment of autoimmune disorders such as RA.

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类风湿性关节炎患者血液中提取的单核细胞中 Tityus sp.的免疫调节作用。
背景:类风湿性关节炎的病理生理机制是由自体反应性淋巴细胞和促炎介质相互作用产生的促炎环境引起的。目前缓解疾病进展的策略会产生不良影响,因此需要新的治疗策略和分子靶点来治疗这种疾病。在这种情况下,有证据表明蝎子毒液可以调节免疫反应和一些重要的细胞机制,具有药理学意义。为了评估 Tityus sp.(一种可能与 Tityus metuendus 接近的新物种)毒液的免疫调节作用,研究人员将被诊断患有 RA 的妇女的外周血单核细胞与对照组的细胞进行了比较:方法:以确诊为 RA 的 10 名妇女为样本,与性别和年龄相匹配的对照组进行病例对照研究。结果:Tityus sp.毒液浓度为 252 和 126 µg/mL 时,可降低 RA 患者和健康对照组 CD3+、CD3+CD4+ 和 CD3+CD8+ 细胞亚群的增殖百分比。然而,该毒液并未引起细胞活化标志物百分比的显著差异。与未经处理的患者和对照组细胞相比,浓度为 252 µg/mL 的毒液会导致 IL-10 减少。结论:Tityus sp.的毒液是CD4和CD8 T淋巴细胞中具有免疫调节能力的分子的潜在来源。这一结果指导了毒液特征研究,以确定对 T 淋巴细胞具有免疫调节能力的药理靶点,从而加强对治疗自身免疫性疾病(如 RA)的研究。
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来源期刊
CiteScore
4.80
自引率
8.30%
发文量
39
审稿时长
6-12 weeks
期刊介绍: Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.
期刊最新文献
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