Bio-Calcium from Skipjack Tuna Frame Attenuates Bone Loss in Ovariectomy-Induced Osteoporosis Rats.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL Marine Drugs Pub Date : 2024-10-16 DOI:10.3390/md22100472

Jirakrit Saetang, Acharaporn Issuriya, Watcharapol Suyapoh, Peerapon Sornying, Krisana Nilsuwan, Soottawat Benjakul

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Abstract

Bio-calcium derived from fish frames may offer several advantages for osteoporosis prevention. This study aimed to evaluate the effects of bio-calcium derived from skipjack tuna frames on bone loss in ovariectomized rats. Tuna bio-calcium was prepared through enzymatic hydrolysis, defatting, bleaching, and grinding processes. The bioavailability of calcium was tested using the Caco-2 cell monolayer model, showing that 13% of tuna bio-calcium was absorbed, compared to 10% for calcium carbonate. Rats were divided into the five following groups: (1) OVX, (2) sham-operated, (3), OVX + estrogen-treated (4) OVX + calcium carbonate-treated, and (5) OVX + tuna bio-calcium-treated. All groups were raised for eight weeks. Tuna bio-calcium was able to increase BV/TV by 26% in the femur and 29% in the tibia, compared to 13% and 17% in the OVX group, respectively. Trabecular thickness in the femur upsurged to 360 µm in the tuna group, while a thickness of 290 µm was observed in the control. Additionally, osteoclast numbers were reduced to 5 N.Oc/mm in the femur and 6 N.Oc/mm in the tibia in the tuna group, compared to 35 and 45 N.Oc/mm in the control. Overall, tuna bio-calcium effectively prevented bone loss and can serve as a promising natural alternative for managing osteoporosis.

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鲣鱼框架中的生物钙可减轻卵巢切除术诱发的骨质疏松症大鼠的骨质流失。

从鱼框中提取的生物钙在预防骨质疏松症方面可能具有多种优势。本研究旨在评估从鲣鱼框中提取的生物钙对卵巢切除大鼠骨质流失的影响。金枪鱼生物钙通过酶水解、脱脂、漂白和研磨过程制备而成。使用 Caco-2 细胞单层模型测试了钙的生物利用率，结果显示金枪鱼生物钙的吸收率为 13%，而碳酸钙的吸收率为 10%。大鼠被分为以下五组：（1）卵巢切除术组；（2）假手术组；（3）卵巢切除术+雌激素处理组；（4）卵巢切除术+碳酸钙处理组；（5）卵巢切除术+金枪鱼生物钙处理组。所有组均饲养八周。金枪鱼生物钙能使股骨和胫骨的BV/TV分别增加26%和29%，而OVX组分别为13%和17%。金枪鱼组的股骨骨小梁厚度增至 360 微米，而对照组的厚度为 290 微米。此外，金枪鱼组股骨和胫骨的破骨细胞数量分别减少到 5 N.Oc/mm 和 6 N.Oc/mm ，而对照组分别为 35 N.Oc/mm 和 45 N.Oc/mm。总之，金枪鱼生物钙可有效防止骨质流失，是治疗骨质疏松症的一种很有前途的天然替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.