A New Real-Time PCR Test (Flora Select™) and Nugent Score for the Diagnosis of Bacterial Vaginosis During Pregnancy.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-10-21 DOI:10.3390/microorganisms12102110
Hideto Yamada, Shigeki Shimada, Hajime Ota, Yuta Kobayashi, Yoshiyuki Fukushi, Shinichiro Wada, Soromon Kataoka
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Abstract

This prospective cohort study aimed to evaluate the performance of Flora select™ (FS), a newly developed real-time PCR test, for the assessment of the vaginal microbiome during early pregnancy. Five hundred and fifty-six pregnant women underwent examinations of FS, Nugent score-a Gram-staining scoring system for the diagnosis of bacterial vaginosis (BV)-and conventional bacterial culture between 8 weeks and 12 gestational weeks. Nugent scores of 0-3, 4-6, and ≥7 were found in 469 (84.2%), 41 (7.4%), and 47 (8.5%) of the women, respectively. Relative dominance rates of Lactobacillus species of high (≥80% medium (50%≤, <80%), and low (0.1≤, <50%), and no detection (<0.1%) were 63.0%, 8.8%, 17.1%, and 11.2%, respectively. Gardnerella, Prevotella, Atopobium, Streptococcus, Ureaplasma, and Mycoplasma species were detected in 23.9%, 17.6%, 17.1%, 7.0%, 23.0%, and 4.9% of the women, respectively. Gardnerella species were detected in all women with Nugent scores ≥7 and Ureaplasma were detected in 40.4% of them. BV-associated bacterial species were also detected in 70.7% of women with Nugent scores of 4-6. Gardnerella, Prevotella, Atopobium, Streptococcus, Ureaplasma, and Mycoplasma species were highly prevalent in women with Nugent scores ≥4 or Lactobacillus species <50%. FS detected Gardnerella, Prevotella, and Atopobium species more effectively than conventional bacterial culture. FS could determine relative dominance rates of Lactobacillus species in the vaginal microbiome, and simultaneously detect four kinds of BV-associated bacteria, Ureaplasma and Mycoplasma species. Therefore, FS may be clinically useful for the screening of the vaginal microbiome during pregnancy to prevent preterm birth and for the assessment of the vaginal microbiome after BV treatments.

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用于诊断妊娠期细菌性阴道病的新型实时 PCR 检验(Flora Select™)和 Nugent 评分。
这项前瞻性队列研究旨在评估 Flora select™ (FS) 的性能,这是一种新开发的实时 PCR 检测方法,用于评估孕早期的阴道微生物群。556 名孕妇在妊娠 8 周至 12 周期间接受了 FS、Nugent 评分(诊断细菌性阴道病(BV)的革兰氏染色评分系统)和传统细菌培养的检查。469 名(84.2%)、41 名(7.4%)和 47 名(8.5%)妇女的 Nugent 评分分别为 0-3、4-6 和≥7 分。在 23.9%、17.6%、17.1%、7.0%、23.0% 和 4.9%的妇女中分别检测到乳酸杆菌属高(≥80%)、中(50%≤)、加德纳菌属、普雷沃特氏菌属、阿托波菌属、链球菌属、解脲支原体属和支原体属的相对优势率。在所有 Nugent 评分≥7 分的妇女中都检测到了加德纳菌,其中 40.4% 的妇女检测到了解脲支原体。在 Nugent 评分为 4-6 分的妇女中,70.7% 的人也检测到了与 BV 相关的细菌种类。加德纳菌、普雷沃特氏菌、阿托波菌、链球菌、解脲支原体和支原体菌种在努根特评分≥4分的妇女中高发,或乳酸杆菌菌种加德纳菌、普雷沃特氏菌和阿托波菌菌种比传统细菌培养更有效。FS 能确定乳杆菌在阴道微生物组中的相对优势率,并能同时检测四种 BV 相关细菌、解脲支原体和支原体。因此,FS 可用于妊娠期阴道微生物组的筛查以预防早产,也可用于 BV 治疗后阴道微生物组的评估。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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