Insertion Sequences within Oxacillinases Genes as Molecular Determinants of Acinetobacter baumannii Resistance to Carbapenems-A Pilot Study.

IF 4.1 2区生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-10-12 DOI:10.3390/microorganisms12102057

Dagmara Depka, Tomasz Bogiel, Mateusz Rzepka, Eugenia Gospodarek-Komkowska

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Abstract

Carbapenem-resistant Acinetobacter baumannii is one of the major problems among hospitalized patients. The presence of multiple virulence factors results in bacteria persistence in the hospital environment. It facilitates bacterial transmission between patients, causing various types of infections, mostly ventilator-associated pneumonia and wound and bloodstream infections. A. baumannii has a variable number of resistance mechanisms, but the most commonly produced are carbapenem-hydrolyzing class D β-lactamases (CHDLs). In our study, the presence of bla_OXA-23, bla_OXA-40 and bla_OXA-51 genes was investigated among 88 clinical isolates of A. baumannii, including 53 (60.2%) strains resistant to both carbapenems (meropenem and imipenem) and 35 (39.8%) strains susceptible to at least meropenem. Among these bacteria, all the isolates carried the bla_OXA-51 gene. The bla_OXA-23 and bla_OXA-40 genes were detected in two (5.7%) and three (8.6%) strains, respectively. Among the OXA-23 carbapenemase-producing A. baumannii strains (n = 55), insertion sequences (ISAba1) were detected upstream of the bla_OXA-23 gene in fifty-two (94.5%) carbapenem-resistant and two (3.6%) meropenem-susceptible isolates. A. baumannii clinical strains from Poland have a similar antimicrobial resistance profile as those worldwide, with the presence of ISAba1 among bla_OXA-23-positive isolates also being quite common. Carbapenem resistance among A. baumannii strains is associated with the presence of CHDLs, especially when insertion sequences are present.

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作为鲍曼不动杆菌对碳青霉烯类耐药性分子决定因素的氧青霉素酶基因内插入序列--一项试验性研究。

耐碳青霉烯类鲍曼不动杆菌是住院病人的主要问题之一。多种毒力因子的存在导致细菌在医院环境中持续存在。它有利于细菌在病人之间传播，导致各种类型的感染，主要是呼吸机相关性肺炎、伤口和血流感染。鲍曼不动杆菌有多种耐药机制，但最常产生的是碳青霉烯水解D类β-内酰胺酶（CHDLs）。在我们的研究中，我们调查了 88 株临床分离的鲍曼不动杆菌中是否存在 blaOXA-23、blaOXA-40 和 blaOXA-51 基因，其中包括 53 株（60.2%）对两种碳青霉烯类（美罗培南和亚胺培南）均耐药的菌株和 35 株（39.8%）至少对美罗培南耐药的菌株。在这些细菌中，所有分离菌株都携带 blaOXA-51 基因。在两株（5.7%）和三株（8.6%）菌株中分别检测到 blaOXA-23 和 blaOXA-40 基因。在产生 OXA-23 碳青霉烯酶的鲍曼不动杆菌菌株（n = 55）中，52 株（94.5%）耐碳青霉烯酶的分离株和 2 株（3.6%）对美罗培南敏感的分离株中检测到 blaOXA-23 基因上游的插入序列（ISAba1）。波兰的鲍曼尼氏菌临床菌株具有与全球类似的抗菌药耐药性特征，在 blaOXA-23 阳性的分离株中，ISAba1 的存在也相当普遍。鲍曼不动杆菌菌株的碳青霉烯耐药性与 CHDLs 的存在有关，尤其是在存在插入序列的情况下。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.