Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective

IF 4.5 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS New biotechnology Pub Date : 2024-10-22 DOI:10.1016/j.nbt.2024.10.002
{"title":"Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective","authors":"","doi":"10.1016/j.nbt.2024.10.002","DOIUrl":null,"url":null,"abstract":"<div><div>Plants face multiple challenges from environmental pollutants and higher emissions of atmospheric CO<sub>2</sub>. Therefore, a hydroponic-based experiment was used to explore the combined effects of elevated [CO<sub>2</sub>] (700 ppm) and exogenous cyanide (CN<sup>−</sup>) (3.0 mg CN/L) on rice seedlings under nitrogen deficiency, utilizing metabonomic and transcriptomic analysis. Elevated [CO<sub>2</sub>] significantly improved the growth of CN<sup>−</sup>-treated rice seedlings compared to those with ambient [CO<sub>2</sub>] (350 ppm), and it also significantly affected CN<sup>−</sup> assimilation. Transcriptome analysis revealed distinct impacts on differentially expressed genes (DEGs) across treatments and tissues. KEGG analysis showed variability in DEGs enriched in amino acid (AA) and energy metabolism pathways due to elevated [CO<sub>2</sub>] and CN<sup>−</sup>. Metabonomic indicated that higher input of [CO<sub>2</sub>] and exogenous CN<sup>−</sup> more severely impacted energy metabolism elements than the individual species of AAs. Positive synergistic effects of elevated [CO<sub>2</sub>] and CN<sup>−</sup> were observed for glutamine and asparagine in shoots, and methionine in roots, wherein negative effects were noted for phenylalanine in shoots, and phenylalanine, valine, and alanine in roots. Meanwhile, positive effects on fumarate in shoots and α-ketoglutarate and succinate in roots were also found. Overall, elevated [CO<sub>2</sub>] enhanced growth in CN<sup>−</sup>-treated rice seedlings under nitrogen deficiency by altering AA and energy metabolism. This is the first attempt to provide new evidence of [CO<sub>2</sub>]-based gaseous fertilization as an energy-saving strategy for rice plants fed with biodegradable N-containing pollutants as a supporting N source under N deficient conditions.</div></div>","PeriodicalId":19190,"journal":{"name":"New biotechnology","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1871678424005521","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Plants face multiple challenges from environmental pollutants and higher emissions of atmospheric CO2. Therefore, a hydroponic-based experiment was used to explore the combined effects of elevated [CO2] (700 ppm) and exogenous cyanide (CN) (3.0 mg CN/L) on rice seedlings under nitrogen deficiency, utilizing metabonomic and transcriptomic analysis. Elevated [CO2] significantly improved the growth of CN-treated rice seedlings compared to those with ambient [CO2] (350 ppm), and it also significantly affected CN assimilation. Transcriptome analysis revealed distinct impacts on differentially expressed genes (DEGs) across treatments and tissues. KEGG analysis showed variability in DEGs enriched in amino acid (AA) and energy metabolism pathways due to elevated [CO2] and CN. Metabonomic indicated that higher input of [CO2] and exogenous CN more severely impacted energy metabolism elements than the individual species of AAs. Positive synergistic effects of elevated [CO2] and CN were observed for glutamine and asparagine in shoots, and methionine in roots, wherein negative effects were noted for phenylalanine in shoots, and phenylalanine, valine, and alanine in roots. Meanwhile, positive effects on fumarate in shoots and α-ketoglutarate and succinate in roots were also found. Overall, elevated [CO2] enhanced growth in CN-treated rice seedlings under nitrogen deficiency by altering AA and energy metabolism. This is the first attempt to provide new evidence of [CO2]-based gaseous fertilization as an energy-saving strategy for rice plants fed with biodegradable N-containing pollutants as a supporting N source under N deficient conditions.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高浓度二氧化碳促进缺氮水稻的生长和氰化物同化:转录组学和代谢组学视角。
植物面临着环境污染物和大气二氧化碳排放量增加的多重挑战。因此,本研究通过水培实验,利用代谢组学和转录组学分析,探讨了高浓度[CO2](700 ppm)和外源氰化物(CN-)(3.0mg CN/L)对缺氮水稻幼苗的综合影响。与环境[CO2](350 ppm)相比,高浓度[CO2]明显改善了外源氰化物(CN-)处理的水稻秧苗的生长,同时也显著影响了CN-的同化。转录组分析显示,不同处理和组织对差异表达基因(DEGs)的影响各不相同。KEGG 分析表明,[CO2]和 CN- 升高会导致氨基酸(AA)和能量代谢途径中富集的 DEGs 发生变化。代谢组学分析表明,较高的[CO2]和外源 CN- 对能量代谢元素的影响比对单种 AA 的影响更为严重。高浓度[CO2]和 CN- 对幼芽中的谷氨酰胺和天冬酰胺以及根中的蛋氨酸产生了正向协同效应,而对幼芽中的苯丙氨酸以及根中的苯丙氨酸、缬氨酸和丙氨酸产生了负向效应。同时,还发现芽中的富马酸和根中的α-酮戊二酸和琥珀酸具有正效应。总之,高浓度[CO2]可通过改变 AA 和能量代谢,促进缺氮条件下经 CN 处理的水稻幼苗的生长。这是首次尝试提供新的证据,证明以[CO2]为基础的气态施肥是缺氮条件下以可生物降解的含氮化合物作为辅助氮源喂养水稻植株的一种节能策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
期刊最新文献
Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production Impact of hydromechanical stress on CHO cells’ metabolism and productivity: Insights from shake flask cultivations with online monitoring of the respiration activity Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses Corrigendum to “Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control” [New Biotechnol 79 (2024) 1–19]
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1