Clinical significance of blood cell ratios in healthy and sick Leishmania infantum-seropositive dogs.

IF 3.5 2区医学 Q1 PARASITOLOGY Parasites & Vectors Pub Date : 2024-10-23 DOI:10.1186/s13071-024-06522-z

Giulia Donato, Marta Baxarias, Laia Solano-Gallego, Icíar Martínez-Flórez, Cristina Mateu, Maria Grazia Pennisi

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Abstract

Background: The accuracy of blood cell ratios (BCRs) as cost-effective and easily accessible diagnostic and prognostic markers of inflammatory conditions has been investigated in veterinary medicine in recent years.

Methods: Neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), and platelet-to-lymphocyte (PLR) ratios were studied in 195 dogs clinically evaluated and tested for anti-Leishmania infantum (Li) antibodies (Li-seronegative (Li^-), n = 10; Li-seropositive clinically healthy (Li⁺_healthy), n = 100; Li-seropositive with clinical and/or clinicopathological abnormalities (Li⁺_sick), n = 85). The Li⁺_sick dogs were classified in LeishVet stages IIa/IIb (Li⁺_IIa/IIb) (n = 66) and III/IV (Li⁺_III/IV) (n = 19). BCR relationships with LeishVet clinical stage, antibody levels, and serum protein electrophoretic fraction concentrations were investigated.

Results: Higher NLR values were found in Li⁺, Li⁺_healthy, and Li⁺_IIa/IIb sick dogs compared to Li^- dogs (P < 0.001). Higher NLR and MLR were found in Li⁺_sick (NLR, P < 0.001; MLR, P = 0.034) and Li⁺_III/IV dogs (NLR, P < 0.001; MLR, P = 0.005) compared to Li^- dogs, and in Li⁺_III/IV dogs (NLR, P = 0.002; MLR, P < 0.001) compared to Li⁺_healthy. All three BCRs were higher in Li⁺_sick (NLR, MLR, P < 0.001; PLR, P = 0.023) and Li⁺_IIa/IIb dogs (NLR P < 0.001; MLR P = 0.001; PLR, P = 0.012) compared to Li⁺_healthy dogs. The BCRs failed to distinguish dogs with moderate (Li⁺_IIa/IIb) and severe or very severe disease (Li⁺_III/IV). BCRs demonstrated weak positive correlations with serum globulin fractions and antibody levels, and weak negative correlations with serum albumin level were found. Li⁺_sick dogs presenting hypoalbuminemia showed higher MLR ratios (P = 0.001) than those with normal albumin values.

Conclusions: This study shows that BCR measures provide useful information for differentiating antibody-positive healthy and sick dogs at diagnosis. Dogs with hypoalbuminemia showed higher MLR values despite monocytosis being very rare.

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健康和患病的幼年利什曼原虫血清阳性犬的血细胞比率的临床意义。

背景：近年来，研究人员对血细胞比值（BCR）作为兽医学中具有成本效益且易于使用的诊断和预后标记物的准确性进行了研究：近年来，兽医学界一直在研究血细胞比值（BCRs）作为炎症性疾病的诊断和预后标记物是否具有成本效益且易于获得：研究了 195 只接受临床评估和抗幼年利什曼原虫（Li）抗体检测的犬只的中性粒细胞对淋巴细胞比率（NLR）、单核细胞对淋巴细胞比率（MLR）和血小板对淋巴细胞比率（PLR）（Li-阴性，n = 10；Li-血清阳性，临床健康（Li+健康），n = 100；Li-血清阳性，临床和/或临床病理异常（Li+患病），n = 85）。Li+病犬分为 LeishVet IIa/IIb 期（Li+IIa/IIb）（n = 66）和 III/IV 期（Li+III/IV）（n = 19）。研究了 BCR 与 LeishVet 临床分期、抗体水平和血清蛋白电泳分数浓度的关系：结果：与Li狗相比，Li+狗、Li+健康狗和Li+Ⅱa/Ⅱb病狗的NLR值更高（P +病狗，NLR，P +Ⅲ/Ⅳ狗，NLR，P -狗；Li+Ⅲ/Ⅳ狗，NLR，P = 0.002；MLR，P +健康狗，MLR，P +Ⅲ/Ⅳ狗）。在 Li+ 病犬（NLR、MLR、P +IIa/IIb 犬（NLR P +healthy 犬）中，所有三个 BCR 值都较高。BCR 无法区分中度疾病（Li+IIa/IIb）和重度或极重度疾病（Li+III/IV）。BCR 与血清球蛋白组分和抗体水平呈弱正相关，与血清白蛋白水平呈弱负相关。出现低白蛋白血症的 Li+ 病犬的 MLR 比率（P = 0.001）高于白蛋白值正常的病犬：本研究表明，BCR 指标可为诊断时区分抗体阳性的健康犬和病犬提供有用信息。尽管单核细胞增多症非常罕见，但低白蛋白血症犬的 MLR 值更高。

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来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.