Cell-nanoparticle stickiness and dose delivery in a multi-model in silico platform: DosiGUI.

IF 7.2 1区 医学 Q1 TOXICOLOGY Particle and Fibre Toxicology Pub Date : 2024-10-24 DOI:10.1186/s12989-024-00607-4
Ermes Botte, Pietro Vagaggini, Ilaria Zanoni, Nicole Guazzelli, Lara Faccani, Davide Gardini, Anna L Costa, Arti Ahluwalia
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Abstract

Background: It is well-known that nanoparticles sediment, diffuse and aggregate when dispersed in a fluid. Once they approach a cell monolayer, depending on the affinity or "stickiness" between cells and nanoparticles, they may adsorb instantaneously, settle slowly - in a time- and concentration-dependent manner - or even encounter steric hindrance and rebound. Therefore, the dose perceived by cells in culture may not necessarily be that initially administered. Methods for quantifying delivered dose are difficult to implement, as they require precise characterization of nanoparticles and exposure scenarios, as well as complex mathematical operations to handle the equations governing the system dynamics. Here we present a pipeline and a graphical user interface, DosiGUI, for application to the accurate nano-dosimetry of engineered nanoparticles on cell monolayers, which also includes methods for determining the parameters characterising nanoparticle-cell stickiness.

Results: We evaluated the stickiness for 3 industrial nanoparticles (TiO2 - NM-105, CeO2 - NM-212 and BaSO4 - NM-220) administered to 3 cell lines (HepG2, A549 and Caco-2) and subsequently estimated corresponding delivered doses. Our results confirm that stickiness is a function of both nanoparticle and cell type, with the stickiest combination being BaSO4 and Caco-2 cells. The results also underline that accurate estimations of the delivered dose cannot prescind from a rigorous evaluation of the affinity between the cell type and nanoparticle under investigation.

Conclusion: Accurate nanoparticle dose estimation in vitro is crucial for in vivo extrapolation, allowing for their safe use in medical and other applications. This study provides a computational platform - DosiGUI - for more reliable dose-response characterization. It also highlights the importance of cell-nanoparticle stickiness for better risk assessment of engineered nanomaterials.

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多模型硅学平台中的细胞-纳米粒子粘性和剂量递送:DosiGUI。
背景:众所周知,纳米粒子在流体中分散时会发生沉淀、扩散和聚集。一旦它们接近细胞单层,根据细胞与纳米粒子之间的亲和力或 "粘性",它们可能会瞬间吸附、缓慢沉降--与时间和浓度有关--甚至遇到立体阻碍而反弹。因此,细胞在培养过程中感知到的剂量不一定是最初给药的剂量。量化给药剂量的方法很难实现,因为它们需要对纳米粒子和暴露情况进行精确表征,还需要复杂的数学运算来处理系统动力学方程。在这里,我们介绍了一种管道和图形用户界面 DosiGUI,用于对细胞单层上的工程纳米粒子进行精确的纳米模拟,其中还包括确定纳米粒子-细胞粘性特征参数的方法:我们评估了 3 种工业纳米粒子(TiO2 - NM-105、CeO2 - NM-212 和 BaSO4 - NM-220)在 3 种细胞系(HepG2、A549 和 Caco-2)中的粘性,随后估算了相应的投放剂量。我们的结果证实,粘度是纳米粒子和细胞类型的函数,其中最粘的组合是 BaSO4 和 Caco-2 细胞。结果还强调,要准确估算投放剂量,就必须严格评估细胞类型与所研究纳米粒子之间的亲和力:准确的体外纳米粒子剂量估算对于体内推断至关重要,可确保纳米粒子在医疗和其他应用中的安全使用。本研究提供了一个计算平台--DosiGUI--用于更可靠的剂量反应表征。它还强调了细胞-纳米颗粒粘性对于更好地评估工程纳米材料风险的重要性。
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来源期刊
CiteScore
15.90
自引率
4.00%
发文量
69
审稿时长
6 months
期刊介绍: Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.
期刊最新文献
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