Utilizing an Ex Vivo Skin Penetration Analysis Model for Predicting Ocular Drug Penetration: A Feasibility Study with Curcumin Formulations.

IF 4.9 3区 医学 Q1 PHARMACOLOGY & PHARMACY Pharmaceutics Pub Date : 2024-10-06 DOI:10.3390/pharmaceutics16101302
Christian Raab, Stefan Brugger, Jara-Sophie Lechner, Geisa Nascimento Barbalho, Taís Gratieri, Priyanka Agarwal, Ilva D Rupenthal, Cornelia M Keck
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Abstract

Objective: This study aimed to investigate the feasibility of using the digital image processing technique, developed to semi-quantitatively study dermal penetration, to study corneal penetration in an ex vivo porcine eye model. Here, we investigated various formulation strategies intended to enhance dermal and corneal bioavailability of the model hydrophobic drug, curcumin.

Methods: Several formulation principles were explored, including oily solutions, oily suspensions, aqueous nanosuspension, micelles, liposomes and cyclodextrins. The dermal penetration efficacy was tested using an ex vivo porcine ear model previously developed at Philipps-Universität Marburg with subsequent digital image processing. This image analysis method was further applied to study corneal penetration using an ex vivo porcine whole-eye model.

Results: For dermal penetration, oily solutions, oily suspensions and nanosuspensions exhibited the least penetration, whereas liposomes and cyclodextrins showed enhanced penetration. Corneal curcumin penetration correlated with dermal penetration, with curcumin loaded into cyclodextrins penetrating the deepest.

Conclusions: Overall, our study suggests that the image analysis method previously developed for ex vivo skin penetration can easily be repurposed to study corneal penetration of hydrophobic drugs.

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利用体内皮肤渗透分析模型预测眼部药物渗透:姜黄素制剂的可行性研究。
研究目的本研究旨在探讨将用于半定量研究皮肤渗透性的数字图像处理技术用于研究猪眼体外模型中角膜渗透性的可行性。在此,我们研究了旨在提高疏水性药物姜黄素的皮肤和角膜生物利用度的各种配方策略:方法:我们探讨了几种制剂原理,包括油性溶液、油性悬浮液、纳米水悬浮液、胶束、脂质体和环糊精。利用马尔堡菲力浦大学之前开发的猪耳体外模型和随后的数字图像处理技术测试了其真皮渗透功效。这种图像分析方法还被进一步应用于使用猪全眼体外模型研究角膜渗透性:结果:在真皮渗透方面,油性溶液、油性悬浮液和纳米悬浮液的渗透率最低,而脂质体和环糊精的渗透率较高。角膜姜黄素渗透率与真皮渗透率相关,环糊精中的姜黄素渗透最深:总之,我们的研究表明,以前开发的用于体外皮肤渗透的图像分析方法可以很容易地重新用于疏水性药物的角膜渗透研究。
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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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