Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Probiotics and Antimicrobial Proteins Pub Date : 2024-10-23 DOI:10.1007/s12602-024-10379-0
Jinping Shi, Yu Lei, Zemin Li, Li Jia, Pengjia He, Qiang Cheng, Zhao Zhang, Zhaomin Lei
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Abstract

We previously observed that supplementation with antimicrobial peptides facilitated the average daily weight gain, net meat, and carcass weights of Holstein bulls. To expand our knowledge of the possible impact of antimicrobial peptides on cecum microbiota, further investigations were conducted. In this study, 18 castrated Holstein bulls with insignificant weight differences and 10 months of age were split randomly into two groups. The control group (CK) was fed a basic diet, whereas the antimicrobial peptide group (AP) was supplemented with 8 g of antimicrobial peptides for 270 days. After slaughter, metagenomic and metabolomic sequencing analyses were performed on the cecum contents. The results showed significantly higher levels of amylase, cellulase, protease, and lipase in the CK than in the AP group (P ≤ 0.05). The levels of β-glucosidase and xylanase (P ≤ 0.05), and acetic and propionic acids (P ≤ 0.01), were considerably elevated in the AP than in the CK group. The metagenome showed variations between the two groups only at the bacterial level, and 3258 bacteria with differences were annotated. A total of 138 differential abundant genes (P < 0.05) were identified in the CAZyme map, with 65 genes more abundant in the cecum of the AP group and 48 genes more abundant in the cecum of the CK group. Metabolomic analysis identified 68 differentially expressed metabolites. Conjoint analysis of microorganisms and metabolites revealed that Lactobacillus had the greatest impact on metabolites in the AP group and Brumimicrobium in the CK group. The advantageous strains of the AP group Firmicutes bacterium CAG:110 exhibited a strong symbiotic relationship with urodeoxycholic acid and hyodeoxycholic acid. This study identified the classification characteristics, functions, metabolites, and interactions of cecal microbiota with metabolites that contribute to host growth performance. Antimicrobial peptides affect the cecal microorganisms, making the use of nutrients more efficient. The utilization of hemicellulose in the cecum of ruminants may contribute more than cellulose to their production performance.

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抗菌肽对盲肠微生物群的改变可提高荷斯坦公牛对营养物质的合理有效利用。
我们以前曾观察到,补充抗菌肽有助于提高荷斯坦公牛的平均日增重、净肉和胴体重量。为了进一步了解抗菌肽对盲肠微生物群可能产生的影响,我们进行了进一步的研究。在这项研究中,18 头体重差异不明显、年龄为 10 个月的阉割荷斯坦公牛被随机分成两组。对照组(CK)饲喂基本饲料,而抗菌肽组(AP)则连续 270 天添加 8 克抗菌肽。屠宰后,对盲肠内容物进行了元基因组和代谢组测序分析。结果显示,CK 组的淀粉酶、纤维素酶、蛋白酶和脂肪酶水平明显高于 AP 组(P ≤ 0.05)。AP组的β-葡萄糖苷酶和木聚糖酶水平(P≤0.05)以及乙酸和丙酸水平(P≤0.01)均明显高于CK组。元基因组仅在细菌水平上显示了两组之间的差异,共注释了 3258 个存在差异的细菌。共有 138 个差异丰度基因(P
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来源期刊
Probiotics and Antimicrobial Proteins
Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY
CiteScore
11.30
自引率
6.10%
发文量
140
期刊介绍: Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.
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