Hb Tacoma by seven HbA1c methods - one with significant interference.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-10-27 DOI:10.1080/00365513.2024.2417383
Anni Mäenpää, Moona Kangastie, Päivikki Kangastupa
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Abstract

Hemoglobin Tacoma is known to potentially interfere HbA1c assays. The variant is common in Finland with prevalence of up to 2% regionally and cases are also reported in areas that have attracted Finnish immigrants, especially in Sweden and North America. Here, we investigated the effect of Hb Tacoma on seven HbA1c methods. 20 non-variant and 20 Hb Tacoma samples were measured with Tina-quant Gen. 3 (immunoassay, considered as reference) and the following point of care instruments: Afinion 2, HbA1c 501 (both utilizing boronate affinity), QuikRead go, cobas b 101, DCA Atellica, and Standard F (all immunoassays). Repeatability was also assessed by measuring both non-variant and Hb Tacoma samples five times each at two different levels. For non-variant samples, the mean relative bias with all methods was < ±4%, whereas for Hb Tacoma samples Standard F had 38% mean relative bias. In absolute bias, the difference was 17 mmol/mol on average and constant through the measured range. For other methods the mean relative bias for Hb Tacoma samples was < ±6%. The repeatability with all methods was similar for non-variant and Hb Tacoma samples and at highest 4.1% (mean CV% of two levels). The observed interference by Standard F is likely due to two-antibody assay design as Hb Tacoma has been shown to result in conformational change. This interference is clinically significant and highlight the need for better controlling and better understanding hemoglobin variants in HbA1c testing.

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用七种 HbA1c 方法检测血红蛋白塔科马--其中一种干扰很大。
众所周知,血红蛋白塔科马可能会干扰 HbA1c 检测。该变异体在芬兰很常见,地区流行率高达 2%,在吸引芬兰移民的地区,尤其是瑞典和北美,也有相关病例报道。在这里,我们研究了 Hb Tacoma 对七种 HbA1c 方法的影响。20 份非变异样本和 20 份 Hb Tacoma 样本是用 Tina-quant Gen:Afinion 2、HbA1c 501(均采用硼酸盐亲和法)、QuikRead go、cobas b 101、DCA Atellica 和 Standard F(均为免疫测定法)。重复性也是通过测量非变异样本和 Hb Tacoma 样本进行评估的,每种样本在两种不同水平下各测量五次。对于非变异样本,所有方法的平均相对偏差为
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来源期刊
CiteScore
3.50
自引率
4.80%
发文量
85
审稿时长
4-8 weeks
期刊介绍: The Scandinavian Journal of Clinical and Laboratory Investigation is an international scientific journal covering clinically oriented biochemical and physiological research. Since the launch of the journal in 1949, it has been a forum for international laboratory medicine, closely related to, and edited by, The Scandinavian Society for Clinical Chemistry. The journal contains peer-reviewed articles, editorials, invited reviews, and short technical notes, as well as several supplements each year. Supplements consist of monographs, and symposium and congress reports covering subjects within clinical chemistry and clinical physiology.
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