Gut microbiota alterations in adolescent idiopathic scoliosis: a comparison study with healthy control and congenital scoliosis.

IF 1.6 Q3 CLINICAL NEUROLOGY Spine deformity Pub Date : 2024-10-22 DOI:10.1007/s43390-024-00988-8
Yinyu Fang, Zhen Tian, Weibiao Li, Dongyue Li, Jie Li, Zongshan Hu, Yong Qiu, Zezhang Zhu, Zhen Liu
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Abstract

Purpose: This study aims to compare the composition of GM isolated from individuals with AIS or congenital scoliosis (CS) and age-matched control (Ctr).

Methods: A total of 48 patients with AIS, 24 patients with CS, and 31 healthy individuals were recruited as the discovery cohort, and 9 pairs of siblings where one was affected by AIS were recruited as the validation cohort. The GM profile was determined with 16S rRNA sequencing, and the alpha-diversity and beta-diversity metrics were performed with Mothur. Linear discriminant analysis (LDA) analysis was performed to identify the enriched species.

Results: The α diversity (Chao1 index) was significantly lower in AIS patients with low BMI (< 18.5) than those with normal BMI. The PcoA analysis showed a trend of clustering of GM in AIS compared to that in Ctr and CS groups (r2 = 0.0553, p = 0.001). METASTAT analysis showed Cellulomonadaceae was significantly enriched in AIS groups compared to CS and Ctr. LDA analysis showed 9 enriched species in AIS patients. Compared to Ctr, two species including Hungatella genus and Bacteroides fragilis were significantly enriched, while the Firmicutes versus Bacteroidetes (F/B) ratio and the Ruminococcus genus were significantly decreased in AIS but not CS groups. The significantly reduced F/B ratio and Ruminococcus genus in AIS were replicated in the validation cohort.

Conclusions: Our study elucidated an association between low BMI and GM diversity in AIS patients. The reduced F/B ratio and Ruminococcus genus in AIS patients were identified and validated in 9 pairs of AIS patients and their unaffected siblings. Our pilot results may help understand the anthropometric discrepancy in these patients and support a possible role of GM in the pathogenesis of AIS.

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青少年特发性脊柱侧弯症的肠道微生物群变化:与健康对照组和先天性脊柱侧弯症的比较研究。
目的:本研究旨在比较从AIS或先天性脊柱侧弯症(CS)患者和年龄匹配对照组(Ctr)分离出的基因组的组成:方法:共招募了48名AIS患者、24名CS患者和31名健康人作为发现队列,并招募了9对兄弟姐妹作为验证队列,其中一人受AIS影响。利用 16S rRNA 测序确定基因组概况,并利用 Mothur 进行α-多样性和β-多样性度量。通过线性判别分析(LDA)确定富集物种:结果:低体重指数的 AIS 患者的 α 多样性(Chao1 指数)明显较低(2 = 0.0553,p = 0.001)。METASTAT 分析表明,与 CS 和 Ctr 相比,AIS 组中的 Cellulomonadaceae 明显富集。LDA分析显示,AIS患者中有9个富集物种。与 Ctr 相比,AIS 组中 Hungatella 属和 Bacteroides fragilis 这两个物种明显富集,而 AIS 组中的 Firmicutes 对 Bacteroidetes(F/B)比率和 Ruminococcus 属则明显下降,CS 组则没有。AIS中F/B比率和反刍球菌属的明显减少在验证队列中得到了复制:我们的研究阐明了低体重指数与 AIS 患者基因组多样性之间的关系。在 9 对 AIS 患者及其未受影响的兄弟姐妹中发现并验证了 AIS 患者的 F/B 比率和反刍球菌属减少。我们的试验结果可能有助于理解这些患者的人体测量差异,并支持基因改造在 AIS 发病机制中可能扮演的角色。
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来源期刊
CiteScore
3.20
自引率
18.80%
发文量
167
期刊介绍: Spine Deformity the official journal of the?Scoliosis Research Society is a peer-refereed publication to disseminate knowledge on basic science and clinical research into the?etiology?biomechanics?treatment?methods and outcomes of all types of?spinal deformities. The international members of the Editorial Board provide a worldwide perspective for the journal's area of interest.The?journal?will enhance the mission of the Society which is to foster the optimal care of all patients with?spine?deformities worldwide. Articles published in?Spine Deformity?are Medline indexed in PubMed.? The journal publishes original articles in the form of clinical and basic research. Spine Deformity will only publish studies that have institutional review board (IRB) or similar ethics committee approval for human and animal studies and have strictly observed these guidelines. The minimum follow-up period for follow-up clinical studies is 24 months.
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