Identification of the proliferative activity of germline progenitor cells in the adult ovary of the bat Artibeus jamaicensis.

IF 1.5 4区 生物学 Q4 CELL BIOLOGY Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-24 DOI:10.1017/S0967199424000364
Tania J Porras-Gómez, Norma Moreno-Mendoza
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Abstract

Until a few years ago, it was assumed that oocyte renewal did not take place in the ovary of adult organisms; however, the existence of germline progenitor cells (GPCs), which renew the ovarian follicular reserve, has now been documented in mammals. Specifically, in the adult ovary of bats, the presence of cells located in the cortical region with characteristics similar to GPCs, called adult cortical germ cells (ACGC), has been observed. One of the requirements that a GPC must fulfil is to be able to proliferate mitotically, so the evaluation of cell proliferation in ACGC is of utmost importance in order to be able to relate them to a parental lineage. Currently, there are several methods to determine cell proliferation, including BrdU labelling or the use of endogenous proliferation markers. Thus, the aim of this work was to evaluate the proliferative activity of ACGC in the adult ovary of the bat Artibeus jamaicensis, using different proliferation markers and correlating these with the protein expression of the transcription factor Oct4 and the germ line marker Ddx4. We found that the expression pattern of the proliferation markers BrdU, PCNA, Ki-67 and pH3 occurs at different times of the cell cycle, so co-localization of two or more of these markers allows us to identify proliferating cells. This allowed us to identify ACGC with proliferative capacity in the adult ovary of A. jamaicensis, suggesting that GPCs renew the follicle reserve during the adult life of the organism.

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鉴定蝙蝠Artibeus jamaicensis成体卵巢中生殖祖细胞的增殖活性。
直到几年前,人们还一直认为成年生物的卵巢中不存在卵母细胞更新;然而,现在哺乳动物中也存在更新卵泡储备的生殖祖细胞(GPC)。具体来说,在蝙蝠的成体卵巢中,观察到位于皮质区的细胞具有类似于生殖祖细胞的特征,这些细胞被称为成体皮质生殖细胞(ACGC)。GPC必须满足的条件之一是能够有丝分裂增殖,因此评估ACGC的细胞增殖情况至关重要,这样才能将它们与亲本血统联系起来。目前,有几种方法可以确定细胞增殖,包括 BrdU 标记或使用内源性增殖标记。因此,这项工作的目的是利用不同的增殖标记物,并将其与转录因子 Oct4 和种系标记物 Ddx4 的蛋白表达相关联,评估蝙蝠成年卵巢中 ACGC 的增殖活性。我们发现,增殖标记物 BrdU、PCNA、Ki-67 和 pH3 的表达模式发生在细胞周期的不同时期,因此,两种或多种标记物的共定位使我们能够识别增殖细胞。这使我们能够识别美洲金枪鱼成年卵巢中具有增殖能力的 ACGC,表明 GPCs 在生物体的成年期更新了卵泡储备。
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来源期刊
Zygote
Zygote 生物-发育生物学
CiteScore
1.70
自引率
5.90%
发文量
117
审稿时长
6-12 weeks
期刊介绍: An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies on gametogenesis through fertilization to gastrulation in animals and humans. The scope has been expanded to include clinical papers, molecular and developmental genetics. The editors will favour work describing fundamental processes in the cellular and molecular mechanisms of animal development, and, in particular, the identification of unifying principles in biology. Nonetheless, new technologies, review articles, debates and letters will become a prominent feature.
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