[Functional Genes and Metabolic Pathways of Nitrogen Metabolism Microorganisms in Lake Sediments:A Case Study of Hongfeng Lake, Guizhou Province].

Q2 Environmental Science Huanjing Kexue/Environmental Science Pub Date : 2024-10-08 DOI:10.13227/j.hjkx.202310134
Qian Liu, Xiao Chen, Yan-Cheng Li, Yu-Han He, Jiang Li
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Abstract

The nitrogen cycle is of great importance for material circulation and energy flow in lake ecosystems. It is driven by microorganisms in lake sediments and can contribute to balancing lake ecosystems. In this study, physical and chemical properties of the sediments sampled from Hongfeng Lake in Guizhou Province were assayed and analyzed using metagenomics to reveal relevant microorganisms, functional genes, metabolic pathways, and their relationships throughout nitrogen metabolism. The results showed that bacteria were dominant, and the top three relative abundant genera were Thiobacillus (16.64%), Rubrivivax(9.43%), and Nitrospira (7.09%). Only six pathways, including nitrogen fixation, nitrification, denitrification, assimilatory nitrate reduction, dissimilatory nitrate reduction, and complete nitrification, were detected in total, of which denitrification and dissimilatory nitrate reduction were the primary processes, but anaerobic ammonia oxidation was not detected. Bacteria and archaea participated in these six pathways, while eukaryotes only functioned in dissimilatory nitrate reduction, denitrification, and complete nitrification. Ammonia nitrogen, nitrate nitrogen, and total phosphorus, as main environmental factors affecting the distribution of functional genes for nitrogen metabolism, differentiated with each other in their respective real-world conditions. A positive correlation (95.04%) was observed between the functional genes and microorganisms, and narG, narZ, and nxrA possessed the highest abundance and the highest host genes. On this basis, these findings are expected to further elucidate the nitrogen cycle of typical karst lakes in Guizhou Province.

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[湖泊沉积物中氮代谢微生物的功能基因与代谢途径:以贵州省红枫湖为例]。
氮循环对湖泊生态系统中的物质循环和能量流动非常重要。氮循环由湖泊沉积物中的微生物驱动,有助于平衡湖泊生态系统。本研究利用元基因组学方法对贵州省红枫湖沉积物的物理和化学性质进行了检测和分析,以揭示整个氮代谢过程中的相关微生物、功能基因、代谢途径及其关系。结果表明,细菌占优势,相对含量前三位的菌属分别为硫杆菌属(16.64%)、红杆菌属(9.43%)和硝化细菌属(7.09%)。总共只检测到固氮、硝化、反硝化、同化作用硝酸盐还原、异化作用硝酸盐还原和完全硝化等六种途径,其中反硝化和异化作用硝酸盐还原是主要过程,但没有检测到厌氧氨氧化。细菌和古细菌参与了这六种途径,而真核生物只参与了硝酸盐还原、反硝化和完全硝化。氨氮、硝态氮和总磷作为影响氮代谢功能基因分布的主要环境因素,在各自的实际条件下存在差异。功能基因与微生物之间存在正相关(95.04%)。narG、narZ 和 nxrA 的丰度最高,宿主基因也最高。在此基础上,这些发现有望进一步阐明贵州省典型岩溶湖泊的氮循环。
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来源期刊
Huanjing Kexue/Environmental Science
Huanjing Kexue/Environmental Science Environmental Science-Environmental Science (all)
CiteScore
4.40
自引率
0.00%
发文量
15329
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