The Altered Proteomic Landscape in Renal Tubular Epithelial Cells under High Oxalate Stimulation.

IF 3.6 3区生物学 Q1 BIOLOGY Biology-Basel Pub Date : 2024-10-11 DOI:10.3390/biology13100814

Sen-Yuan Hong, Bao-Long Qin

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Abstract

Our study aimed to apply a proteomic approach to investigate the molecular mechanisms underlying the effects of oxalate on rat renal tubular epithelial cells. NRK-52E cells were treated with or without oxalate and subjected to quantitative proteomics to identify key proteins and key pathological changes under high oxalate stimulation. A total of 268 differentially expressed proteins (DEPs) between oxalate-treated and control groups were identified, with 132 up-regulated and 136 down-regulated proteins. Functional enrichment analysis revealed that DEPs are associated with oxidative stress, apoptosis, ferroptosis, pro-inflammatory cytokines, vitamin D, and biomineralization. SPP1, MFGE8, ANKS1A, and NAP1L1 were up-regulated in the oxalate-treated cells and the hyperoxaluric stone-forming rats, while SUB1, RNPS1, and DGLUCY were down-regulated in both cases. This altered proteomic landscape sheds light on the pathological processes involved in oxalate-induced renal damage and identifies potential biomarkers and therapeutic targets to mitigate the effects of hyperoxaluria and reduce the risk of CaOx stone formation.

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高草酸盐刺激下肾小管上皮细胞蛋白质组景观的改变

我们的研究旨在应用蛋白质组学方法研究草酸盐对大鼠肾小管上皮细胞影响的分子机制。用或不用草酸盐处理 NRK-52E 细胞，并对其进行定量蛋白质组学研究，以确定高草酸盐刺激下的关键蛋白质和关键病理变化。结果发现，草酸盐处理组和对照组之间共有 268 个差异表达蛋白（DEPs），其中 132 个上调，136 个下调。功能富集分析表明，DEPs 与氧化应激、细胞凋亡、铁凋亡、促炎细胞因子、维生素 D 和生物矿化有关。SPP1、MFGE8、ANKS1A 和 NAP1L1 在草酸盐处理的细胞和高草酸结石大鼠中上调，而 SUB1、RNPS1 和 DGLUCY 则在这两种情况下下调。蛋白质组的这种变化揭示了草酸盐诱导的肾损伤所涉及的病理过程，并确定了潜在的生物标记物和治疗靶点，以减轻高草酸血症的影响并降低形成 CaOx 结石的风险。

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来源期刊

Biology-Basel Biological Science-Biological Science

CiteScore

5.70

自引率

4.80%

发文量

1618

审稿时长

11 weeks

期刊介绍： Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.