A novel broad-spectrum antibacterial and anti-malarial Anopheles gambiae Cecropin promotes microbial clearance during pupation.

IF 5.5 1区医学 Q1 MICROBIOLOGY PLoS Pathogens Pub Date : 2024-10-23 eCollection Date: 2024-10-01 DOI:10.1371/journal.ppat.1012652

Cairé Barreto, Victor Cardoso-Jaime, George Dimopoulos

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Abstract

Anophelinae mosquitoes are exposed to a variety of microbes including Plasmodium parasites that cause malaria. When infected, mosquitoes mount versatile immune responses, including the production of antimicrobial peptides. Cecropins are one of the most widely distributed families of antimicrobial peptides in insects and all previously studied Anopheles members are playing roles in adult mosquito immunity. We have identified and characterized a novel member of the Anopheles gambiae cecropin family, cecropin D (CecD), that is uniquely expressed and immune-responsive at late larval stages to promote microbial clearance through its broad-spectrum antibacterial activity during larval-pupal developmental transition. Interestingly, Cecropin D also exhibited highly potent activity against Plasmodium falciparum sporozoites, the malaria parasite stage that is transmitted from mosquitoes and infects humans and thereby holds promise as a malaria transmission-blocking agent. Finally, we have defined unequivocal cecropin-specific molecular signatures to systematically organize the diversity of the cecropin family in malaria vectors.

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一种新型广谱抗菌和抗疟冈比亚按蚊 Cecropin 能促进蛹化过程中的微生物清除。

按蚊会接触各种微生物，包括导致疟疾的疟原虫寄生虫。受到感染时，蚊子会产生多种免疫反应，包括产生抗菌肽。克氏肽是昆虫中分布最广的抗菌肽家族之一，以前研究的所有按蚊成员都在成蚊免疫中发挥作用。我们发现并鉴定了冈比亚按蚊麦角蛋白家族的一个新成员--麦角蛋白 D（CecD），它在幼虫晚期具有独特的表达和免疫反应能力，在幼虫-蛹发育过渡期通过其广谱抗菌活性促进微生物清除。有趣的是，Cecropin D 对恶性疟原虫孢子虫也表现出极强的活性，恶性疟原虫孢子虫是由蚊子传播并感染人类的疟原虫阶段，因此有望成为一种疟疾传播阻断剂。最后，我们确定了明确的麦角蛋白特异性分子特征，系统地组织了疟疾病媒中麦角蛋白家族的多样性。

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来源期刊

PLoS Pathogens MICROBIOLOGY-PARASITOLOGY

自引率

3.00%

发文量

598

期刊介绍： Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.