Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae.

IF 1.4 4区医学 Q4 INFECTIOUS DISEASES Journal of Infection in Developing Countries Pub Date : 2024-09-30 DOI:10.3855/jidc.17519

Boukaré Kaboré, Ganamé A Ouédraogo, Henri S Ouédraogo, Hama Cissé, Oumarou Zongo, Koudbi J Zongo, Boukaré Zeba, Idrissa Sanou, Aly Savadogo

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Abstract

Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen for which new antibiotics are urgently needed. This study aimed to use molecular tools to identify and examine antibiotic resistance in clinical strains of K. pneumoniae.

Methodology: A total of 15 unduplicated K. pneumoniae strains isolated from patient samples with multidrug-resistant (MDR) profiles were subjected to polymerase chain reaction (PCR) to amplify the most common carbapenem resistance genes. (GTG)5 PCR and phylogenetic analysis were performed to identify the genetic relationship between the strains.

Results: All strains yielded a (GTG)5-PCR profile, and this allowed us to group these strains into 8 groups according to the size and number of characteristic bands. Phylogenetic analysis was done using the free software UPGMA and a single bacterial clone with a correlation coefficient of over 97% was identified. New Delhi metallo-beta-lactamase NDM-like (blaNDM) carbapenem resistance genes were detected in three strains of K. pneumoniae, which represented a resistance rate of 20%. However, carbapenemases type A [Klebsiella pneumoniae carbapenemase (KPC) and imipenem-hydrolysing beta-lactamase (IMI), type D [oxacillinase-48 (OXA-48)], and other metallo-β-lactamase [Verona integron-encoded metallo-beta-lactamase (VIM), and enzyme active on imipenem (IMP)] were not detected.

Conclusions: We identified and grouped the blaNDM resistance genes of Klebsiella pneumonia strains.

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肺炎克雷伯氏菌临床菌株碳青霉烯酶基因的鉴定和系统发育分析。

导言：肺炎克雷伯氏菌是一种包裹型革兰氏阴性菌，是全球医疗机构中众多感染的罪魁祸首，经常被分离出来。世界卫生组织已将肺炎克雷伯菌列为严重的抗生素耐药细菌病原体，急需新的抗生素来治疗。本研究旨在利用分子工具鉴定和检查肺炎克氏菌临床菌株的抗生素耐药性：方法：对从具有多重耐药性（MDR）的患者样本中分离出的 15 株不重复的肺炎克氏菌进行聚合酶链式反应（PCR），以扩增最常见的碳青霉烯类耐药基因。进行了 (GTG)5 PCR 和系统发育分析，以确定菌株之间的遗传关系：结果：所有菌株都产生了 (GTG)5-PCR 图谱，因此我们可以根据特征条带的大小和数量将这些菌株分为 8 组。我们使用免费软件 UPGMA 进行了系统发育分析，并确定了一个相关系数超过 97% 的单一细菌克隆。在三株肺炎克氏菌中检测到了新德里金属-β-内酰胺酶 NDM 样（blaNDM）碳青霉烯耐药基因，耐药率为 20%。但未检测到 A 型碳青霉烯酶[肺炎克雷伯菌碳青霉烯酶（KPC）和亚胺培南水解β-内酰胺酶（IMI）]、D 型碳青霉烯酶[奥沙西林酶-48（OXA-48）]和其他金属-β-内酰胺酶[维罗纳整合子编码的金属-β-内酰胺酶（VIM）和亚胺培南活性酶（IMP）]：我们对肺炎克雷伯菌株的 blaNDM 耐药基因进行了鉴定和分组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection in Developing Countries INFECTIOUS DISEASES-

CiteScore

3.70

自引率

5.30%

发文量

239

审稿时长

4-8 weeks

期刊介绍： The Journal of Infection in Developing Countries (JIDC) is an international journal, intended for the publication of scientific articles from Developing Countries by scientists from Developing Countries. JIDC is an independent, on-line publication with an international editorial board. JIDC is open access with no cost to view or download articles and reasonable cost for publication of research artcles, making JIDC easily availiable to scientists from resource restricted regions.