Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights.

IF 3.8 3区医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2024-10-12 DOI:10.3390/v16101603

Talita Émile Ribeiro Adelino, Sílvia Helena Sousa Pietra Pedroso, Maurício Lima, Luiz Marcelo Ribeiro Tomé, Natália Rocha Guimarães, Vagner Fonseca, Paulo Eduardo de Souza da Silva, Keldenn Melo Farias Moreno, Ana Cândida Araújo E Silva, Náthale Rodrigues Pinheiro, Carolina Senra Alves de Souza, Luiz Carlos Junior Alcantara, Marta Giovanetti, Felipe Campos de Melo Iani

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Abstract

This study examines a case involving a 7-year-old child who developed dengue symptoms following Qdenga vaccination. Despite initial negative diagnostic results, molecular analysis confirmed an infection with DENV4. Next-generation sequencing detected viral RNA from both DENV2 and DENV4 serotypes, which were identified as vaccine-derived strains using specific primers. Phylogenetic analysis further confirmed that these sequences belonged to the Qdenga vaccine rather than circulating wild-type viruses. This case underscores the critical need for precise diagnostic interpretation in vaccinated individuals to avoid misdiagnosis and to strengthen public health surveillance. A comprehensive understanding of vaccine-induced viremia is essential for refining dengue surveillance, improving diagnostic accuracy, and informing public health strategies in endemic regions.

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探索接种疫苗者的登革热感染：初步分子诊断和测序洞察。

本研究探讨了一例 7 岁儿童接种 Qdenga 疫苗后出现登革热症状的病例。尽管最初的诊断结果呈阴性，但分子分析证实他感染了 DENV4。下一代测序检测到了 DENV2 和 DENV4 血清型的病毒 RNA，使用特异性引物将其鉴定为疫苗衍生株。系统发育分析进一步证实，这些序列属于Qdenga疫苗，而非流行的野生型病毒。该病例强调了对接种疫苗者进行精确诊断的重要性，以避免误诊并加强公共卫生监测。全面了解疫苗诱导的病毒血症对于完善登革热监测、提高诊断准确性以及为流行地区的公共卫生策略提供信息至关重要。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.